北京中医药大学学报2018，Vol.41Issue(1)：53-59,7.DOI:10.3969/j.issn.1006-2157.2018.01.009
不同泻药致豚鼠结肠黑变病的实验研究
Melanosis of colon induced by different laxatives in guinea pigs: an experimental study
摘要
Abstract
Objective To discuss the pathogenesis of melanosis of colon(MC).Methods SPF guinea pigs were randomly divided into 4 groups: normal group(given water,2 mL n=8), group of Dahuang (Radix et Rhizoma Rhei, Rhubarb; Dahuang group,1.16 g/kg n=16), group of Fanxieye(Folium Sennae,Senna Leaf, Fanxieye group,0.46 g/kg n=16)and Phenolphthalein Tablets group(15.43 mg/kg n=16),and all groups were intragastrically given corresponding medicinal.The half numbor of guinea pigs was executed on the 4thweek and other half were executed on the 8thweek.The status of colonic pigmentation of guinea pigs in each group was observed by direct visual observation and melanin staining.The colonic histopathology of guinea pigs in each group was observed by the method of HE staining.The apoptosis of colon tissue was detected by using terminal-deoxynucleoitidyl transferase mediated nick end labeling(TUNEL), and level of tumor necrosis factor-α(TNF-α)was detected by using enzyme-linked immunosorbent assay(ELISA)in all groups.Results There was MC occurred in varying degrees in Dahuang group,Fanxieye group and Phenolphthalein Tablets group after 4 weeks and 8 weeks(P<0.01).MC aggravated along with time increased,however,there was statistical difference in MC severity only between Fanxieye group and normal group after 4 weeks and 8 weeks(P<0.05). The value of optical density(OD)was significantly higher in Dahuang group, Fanxieye group and Phenolphthalein Tablets group than that in normal group after 4 weeks and 8 weeks(P<0.01), and along with time increased, OD value also increased but had no statistical difference(P>0.05).The level of TNF-αwas higher in Dahuang group than that in normal group after 4 weeks and 8 weeks(P<0.05),and increased significantly along with time increased(P<0.01).The level of TNF-αhad no statistical difference between Fanxieye group and normal group after 4 weeks(P>0.05),was higher in Fanxieye group than that in normal group after 8 weeks(P<0.05), and increased significantly along with time increased(P <0.01).The level of TNF-α had no statistical difference between Phenolphthalein Tablets group and normal group after 4 weeks and 8 weeks(P>0.05),and along with time increased, level of TNF-α also increased but had no statistical difference(P >0.05). Conclusion Dahuang,Fanxieye and Phenolphthalein Tablets all can induce MC in guinea pigs, and the pathogenesis may be related to apoptosis of colonic epithelial cells.But the relationship between MC and level of TNF-αneeds further research.关键词
结肠黑变病/细胞凋亡/肿瘤坏死因子/大黄/番泻叶/果导片/豚鼠Key words
melanosis of colon/apoptosis/tumor necrosis factor-α/Dahuang(Radix et Rhizoma Rhei/Rhubarb)/Fanxieye(Folium Sennae/Senna Leaf)/Phenolphthalein Tablets/guinea pigs分类
医药卫生引用本文复制引用
郁强,刘薇,蒋静,刘仍海..不同泻药致豚鼠结肠黑变病的实验研究[J].北京中医药大学学报,2018,41(1):53-59,7.基金项目
国家自然科学基金资助项目(No.81273757)National Natural Science Foundation of China(No.81273757) （No.81273757）
