北京中医药大学学报2018，Vol.41Issue(1)：60-64,75,6.DOI:10.3969/j.issn.1006-2157.2018.01.010
六味地黄汤含药血清对HK-2细胞PHD2/HIF-1α信号途径的影响
Influence of medicated serum of Liuwei Dihuang Tang on PHD2/HIF-1α signal pathway in HK-2 cells
摘要
Abstract
Objective To investigate the effect of PHD2/HIF-1 signaling pathway on the renal interstitial fibrosis,and the intervention effect of medicated serum of Liuwei Dihuang Tang on HK-2 cells induced by hypoxia.Methods A total of 40 SPF male SD rats were divided into blank group and medicated group(each n=20).The blank group was orally given normal saline 〔10 mL/(kg· d)〕 and medicated group was orally given Liuwei Dihuang Tang for 1 week continuously, and then blood samples were collected from rat abdominal aorta for separating serum after centrifugation.HK-2 cells were cultured in vitro.HK-2 cells were divided into blank control group(10% of fetal bovine serum), normal group (10%of normal serum),CoCl2group(150 μmol/L of CoCl2+10% of normal serum)and medicated serum group(150 μmol/L of CoCl2+10% of medicated serum of Liuwei Dihuang Tang).HK-2 cells were cultivated in vitro and treated in all groups for 24 h,and then the influence of medicated serum of Liuwei Dihuang Tang on protein levels and mRNA expressions of PHD 2, HIF-1αand connective tissue growth factor(CTGF)in HK-2 cells were detected,respectively,by using Western blotting method and reverse transcription polymerase chain reaction(RT-PCR).Results Compared with normal group, the protein level and mRNA expression of PHD 2 in HK-2 cells all decreased(P <0.05), while protein levels of HIF-1αand CTGF and mRNA expressions of HIF-1αand CTGF increased(P<0.05)in CoCl2 group.Compared with CoCl 2group, the protein level and mRNA expression of PHD2 increased(P<0.05),and those of HIF-1αand CTGF decreased(P<0.05)in medicated serum group.Conclusion PHD2/HIF-1αsignal pathway takes part in RIF, and Liuwei Dihuang Tang can inhibit RIF through up-regulating PHD2 expression,improving HIF-1αdegradation and down-regulating CTGF expression.关键词
HK-细胞/肾间质纤维化/脯氨酸羟化酶2/缺氧诱导因子1α/结缔组织生长因子/六味地黄汤含药血清/大鼠Key words
HK-2 cells/renal interstitial fibrosis/proline hydroxylase 2/hypoxia-inducible factor 1α/connective tissue growth factor/medicated serum of Liuwei Dihuang Tang(Six-Ingredient Rehmannia Decoction)/rats分类
医药卫生引用本文复制引用
唐群,吴华,刘春燕,罗琳,易纯,徐文峰,庄高建..六味地黄汤含药血清对HK-2细胞PHD2/HIF-1α信号途径的影响[J].北京中医药大学学报,2018,41(1):60-64,75,6.基金项目
国家自然科学基金项目(No.81503442,No.81603470),湖南省教育厅科研项目(No.17B197,No.16B196)National Natural Science Foundation of China(No.81503442、No.81603470), Education Department of Hunan Province(No.17B197, No. 16B196) （No.81503442,No.81603470）
