北京中医药大学学报2018，Vol.41Issue(1)：76-82,7.DOI:10.3969/j.issn.1006-2157.2018.01.013
扶正化瘀片调节肝纤维化miR-122基因相关因子表达研究
Expression of miR-122-related factors of liver fibrosis regulated by Fuzheng Huayu Tablet
摘要
Abstract
Objective To investigate softening-hardness dissipating-binds effects of Fuzheng Huayu (reinforcing-healthy qi resolving-stasis)Tablet combined with anti-virus therapy on liver fibrosis, and relationship between these effects and regulation of pathway of miR-122-interleukin-10-reactive oxygen species(miR-122/IL-10/ROS).Methods The patients with chronic hepatitis and liver fibrosis(n=85)were chosen from Aug.2015 to Aug.2016,and then divided randomly into test group(n=45)and control group(n=40).The control group was treated with entecavir(0.5 mg/d)and monoammonium glycyrrhizinate(0.1 g/d),and test group, additionally with Fuzheng Huayu Tablet(4.5 g/d)for 48 weeks.After treatment,ISHAK fibrosis score was reviewed through routine pathology, and activities of serum superoxide dismutase(SOD)and ROS were detected by using ELISA.The expressions of miR-122 mRNA and IL-10 mRNA were detected by using RT-PCR, and content of serum inflammatory factors of liver fibrosis, including procollagen III N-terminal peptide(PCIIINP), type IV collagen(IV-C), hyaluronidase(HA)and laminin(LN),were detected by using immunoturbidimetry.Results ISHAK fibrosis score had no significant difference between 2 groups before treatment,and decreased in 2 groups after treatment,which was superior in test group to that in control group(P<0.05).The content of serum inflammatory factors of liver fibrosis all decreased in 2 groups after treatment,and the decreases of PCIIINP and HA were more significant in test group compared with control group(P <0.05).The activities of ROS and SOD had no difference in 2 groups before treatment,and ROS decreased in 2 groups after treatment.The inhibitory effect on ROS was better in test group than that in control group after treatment(P<0.05), and SOD increase was effectively relieved in test group after treatment(P <0.05).The expressions of miR-122 mRNA and IL-10 mRNA all decreased in 2 groups after treatment, which was more significant in test group compared with control group(P<0.05).Conclusion Fuzheng Huayu Tablet reduces directly the levels of collagen and HA, degrades abnormal deposition of extracellular matrix,and regulates dynamic balance of collagen,and it antagonizes effectively the genetic expressions of IL-10 and miR-122,relieves inflammatory disorders and controls progress of histology.关键词
肝纤维化/微小基因-122/转化生长因子/白细胞介素10/扶正化瘀Key words
liver fibrosis/miR-122/transforming growth factor/interleukin-10/reinforcing healthy qi and resolving stasis分类
医药卫生引用本文复制引用
张巍,邵明亮,张海丛,吴宗耀,苗同国,郑欢伟,谢星童,王蕊..扶正化瘀片调节肝纤维化miR-122基因相关因子表达研究[J].北京中医药大学学报,2018,41(1):76-82,7.基金项目
国家十二五科技重大专项项目(No.2014ZX10005001-009),河北省科技计划项目(No.17277730D)National Science and Technology major projects(No.2014ZX10005001 －009),Science and Technology Projects of Hebei Province(No. 17277730D) （No.2014ZX10005001-009）
