北京中医药大学学报2018，Vol.41Issue(2)：102-109,8.DOI:10.3969/j.issn.1006-2157.2018.02.003
肝郁证雌性大鼠的动情期变化及柴胡疏肝散的防治作用
Changes of estrus in female rats with liver-stagnation pattern and effects of Chaihu Shugan San on its prevention and treatment
摘要
Abstract
Objective To investigate the changes of estrous cycle and the preventive and therapeutic effects of Chaihu Shugan San(Radix bupleuri liver-soothing Powder,CHSGS)in female rats with liver-stagnation pattern.Methods Female rats were randomly divided into normal group, liver-stagnation group,high-dose CHSGS group(H group), and low-dose CHSGS group(L group)(n =10 in each group).Liver stagnation pattern was established by using chronic restraint stress for 4 weeks.After mod-eling for 14 days,rats in the H and L groups were given the investigated medicine at the dose of 1.26 g/kg and 0.31 g/kg(equivalent to 2 and 0.5 times of human dosage)respectively, once a day for 2 weeks.Rats in the model group and the normal group were given the same amount of distilled water.At week 2 and week 4,sucrose preference test(SPT)was conducted; At week 4, open field test(OFT) was performed.Rat estrous cycles were observed by using vaginal smears.General observation was used throughout the experiment.Results At week 2, compared with the normal group, the model group showed a state of hyperactivity,irritability,reduced and slower mobility,and reduced food-intake.SPT were higher at week 2 but decreased at week 4(P<0.01).Estrous cycle disorders were also reduced (P<0.01).Duration in central zone(Index S),cubicle-crossing counts(Index C)and vertical move-ments(Index N)decreased in OFT test(P<0.01).Compared with the model group, the activities of the rats in the H group were more similar to the control group,with the duration in the central zone and vertical movements higher than those in the model group(P<0.01)and SPT decreased(P<0.01). Disordered estrous cycles decreased in H group(P<0.01).The above-mentioned changes were not ob-served in the L group(P>0.05).Conclusion Behavior changes with estrous cycle disorders were ob-served in female rat with liver-stagnation model established by chronic restraints stress.Chaihu Shugan San appeared to be effective in relieving the symptoms and estrous cycle disorders.High-dose CHSGS was superior to low-dose CHSGS.关键词
肝郁证/证候模型/动情周期/柴胡疏肝散/雌鼠Key words
liver-stagnation/model/female rat/estrous cycle/Chaihu Shugan Powder分类
医药卫生引用本文复制引用
张媛凤,钱梦,刘碧原,谢鸣..肝郁证雌性大鼠的动情期变化及柴胡疏肝散的防治作用[J].北京中医药大学学报,2018,41(2):102-109,8.基金项目
国家自然科学基金资助项目(No.81173193)General Program National Natural Science Foundation of China(No.81173193) （No.81173193）
