北京中医药大学学报2018，Vol.41Issue(2)：110-117,8.DOI:10.3969/j.issn.1006-2157.2018.02.004
高尿酸血症大鼠的肠道尿酸排泄研究
Intestinal uric acid excretion in rats with hyperuricemia
摘要
Abstract
Objective To explore the pathological mechanism of hyperuricemia by observing the altera-tion of intestinal uric acid excretion and intestinal transporter ABCG 2 expression in rats with hyperurice-mia induced by fructose.Methods SD male rats(n=24)were randomly divided into normal group and model group according to the weight.The rats in the normal group were given water,and the rats in mod-el group were given 10% fructose solution to establish hyperuricemia model.Levels of serum uric acid (SUA)and fecal uric acid(FUA)were measured every 10 days.The intestinal urate excretion(IUE) level was detected by using in situ perfusion method and HPLC.The location and expression of transport-er ABCG2 in the intestine was analyzed with immunohistochemical staining and semi-quantitative analysis of protein was conducted.Protein expression of transporter ABCG 2 in each part of intestine was further detected by using Western blot.The expression of mRNA of ABCG2 in both groups was determined with RT-qPCR.Results From the 20thto the 40thdays,SUA in the model group was significantly higher than that in normal group.From the 10thto the 30thdays,the FUA in model group was significantly lower than that in normal group.The IUC in model group was significantly lower than that in normal group.Immuno-histochemical staining showed that the intestinal transporter ABCG 2 was located in the intestinal villus and gland,and the analysis of staining showed that the protein expression of ABCG 2 in the jejunum and ileum was significantly lower than those in the normal group.Western blot tests exhibited the same re-sults.RT-qPCR indicated that compared with the normal group, expression of mRNA of ABCG2 in the jejunum and ileum in the model group were markedly lower.Conclusion Fructose could successfully in-duce hyperuricemia in rats.Hyperuricemia induced by fructose presents dysfunction in the intestinal uric acid excretion,which is probably related to the down-regulation of the ABCG2 mRNA level and inhibition of ABCG2 protein level in the jejunum and ileum.关键词
高尿酸血症/肠道排泄/肠道转运蛋白/大鼠Key words
hyperuricemia/intestinal uric acid excretion/intestinal transporter/ABCG2/rats分类
医药卫生引用本文复制引用
王雨,林志健,张冰..高尿酸血症大鼠的肠道尿酸排泄研究[J].北京中医药大学学报,2018,41(2):110-117,8.基金项目
国家自然科学基金资助项目(No.81673618),北京市自然科学基金资助项目(No.7162117)National Natural Science Foundation of China(No.81673618),Beijing Provincial Science Foundation(No.7162117) （No.81673618）
