北京中医药大学学报2018，Vol.41Issue(2)：118-123,6.DOI:10.3969/j.issn.1006-2157.2018.02.005
补肾活血方对薄型子宫内膜干细胞Wnt/β-catenin信号通路的影响
Effects of kidney-supplementing and blood-activating formula on Wnt/β-catenin signal pathway of stem cell of thin endometrium
摘要
Abstract
Objective To investigate the effects of Bushen Huoxue Fang kidney-supplementing and blood-activating formula(BSHXF)on Wnt/β-catenin signal pathway in stem cell of thin endometrium. Methods During the 7thto 9thday after ovulation,100 mg endometrial membrane samples of selected patients with thin endometrium were taken with disposable endometrial sampling device.After washing with saline,the samples was fixed in 4%polyformaldehyde and then separated to culture the endometrial stem cells,and drug serum was prepared.The cultured endometrium stem cells were divided into the control group,High,medium and low dose groups of kidney-supplementing and blood-activating formula (BSHXF)and Western medicine group.After intervention,β-catenin /TCF(T cytokine)and transcrip-tional activity were measured by using double Luciferase Report System;CD34/CD117 protein expression was examined with immunofluorescence; Oct-4 gene was examined with RT-PCR; and ABCG2 protein was measured by using Western blot.Results After transfecting the same dosage of plasmid,there was a significant difference among mid-dose BSHXF group, high-dose BSHXF group, Western medicine group,and control group(P<0.05).CD34/CD117, Oct-4 gene and ABCG2 protein had positive ex-pression in the endometrium stem cells.There was a significant difference in the expression of CD 34 pro-tein between BSHXF groups and control group(P<0.05).There was a significant difference between high-dose BSHXF group and Western medicine group(P<0.01).The expression of CD117 protein in the high-dose group was significantly different from that in the control group(P<0.05),and there was a significant difference between the Western medicine group and the control group(P<0.01).RT-PCR test showed that the expression rate of Oct-4mRNA were significantly different from the interventional groups and the control group(P<0.05).Western blot test showed that the expression rate of ABCG 2 protein in the interventional groups were significantly different from that in the control group(P<0.05). Conclusion Kidney-supplementing and blood-activating formula can increase the fluorescent activity of thin endometrial stem cells, thereby promote the activation of Wnt/β-catenin signal pathway, and in-crease the expression of CD34/CD117,OCT4 gene and ABCG2 protein.关键词
薄型子宫内膜/干细胞/补肾活血方/Wnt/β-catenin信号通路Key words
thin endometrium/endometrium stem cells/kidney-supplementing and blood-activating formula/Wnt/β-catenin signal pathway分类
医药卫生引用本文复制引用
尹晓丹,何军琴,杨维,辛明蔚,薛晓鸥..补肾活血方对薄型子宫内膜干细胞Wnt/β-catenin信号通路的影响[J].北京中医药大学学报,2018,41(2):118-123,6.基金项目
北京市自然科学基金资助项目(No.7164260)Beijing Natural Science Foundation(No.7164260) （No.7164260）
