北京中医药大学学报2018，Vol.41Issue(2)：124-130,7.DOI:10.3969/j.issn.1006-2157.2018.02.006
黄芩素对注意缺陷多动障碍大鼠前额叶皮质AC-cAMP-PKA信号通路的调控作用
Effects of baicalein on AC-cAMP-PKA signaling pathway in prefrontal cortex of rats with attentional deficit hyperactivity disorder
摘要
Abstract
Objective To study the effects of baicalein on spontaneous activity, impulsivity, learning-memorial ability,and the adenylate cyclase(AC)-cyclic adenosine monophosphate(cAMP)-protein ki-nase A(PKA)signaling pathway in prefrontal cortex(PFC)of rats with attention deficit hyperactivity disorder(ADHD).Methods Fifty 4-week-old SHR rats were randomly divided into the model group,methylphenidate(MPH)group(2 mg/kg), and low-dose, mid-dose and high-dose(100, 200, 400 mg/kg)baicalein groups,and WKY rats of the same age were selected as the normal control group(n=10 in each group).Rats were orally gavaged for 4 weeks in the dark-phase.Open field test(OFT),ele-vated plus maze(EPM)and novel object recognition test(NORT)were conducted to evaluate the spon-taneous activity, impulsivity and learning-memorial ability of rats individually at the end of treatment. AC,cAMP and PKA quantity were determined with enzyme-linked immunosorbent(ELISA)method. Results Movement distance in OFT was reduced in high-dose baicalein group and mid-dose baicalein group(P<0.05)afterwards.Vertical movements also decreased in all baicalein groups.Percentage of the times entering the open arms in the EPM was significantly reduced in the mid-dose baicalein group(P<0.05);staying duration in the total period in the EPM was also reduced in all baicalein groups(P<0. 05).The preference index in the testing phase of NORT increased in the mid-dose baicalein group(P<0.05).Levels of AC and cAMP in the PFC increased in all baicalein groups(P<0.05), while tPKA increased in high-dose and mid-dose baicalein group(P<0.05).Conclusion Baicalein could increase the levels of AC,cAMP and PKA in PFC of ADHD rats, which may shed some light to the mechanism how baicalein could relieve the spontaneous activity and impulsivity,improve learning-memorial ability of ADHD rats.关键词
注意缺陷多动障碍/黄芩素/腺苷酸环化酶/环磷酸腺苷/蛋白激酶A/大鼠Key words
attentional deficit hyperactivity disorder/baicalein/AC/cAMP/PKA/rats分类
医药卫生引用本文复制引用
王清,樊晋萍,廖颖钊,陈景,刘华,倪新强..黄芩素对注意缺陷多动障碍大鼠前额叶皮质AC-cAMP-PKA信号通路的调控作用[J].北京中医药大学学报,2018,41(2):124-130,7.基金项目
国家自然科学基金资助项目(No.81503616),广东省科技计划项目(No.2014A020221003),广东省中医药局课题(No.20161218),深圳市科技创新委员会项目(No.JCYJ20160428105935612),深圳市卫生和计划生育委员会项目(No.201505019),海南医学院科研培育基金项目(No.HY2018-34)National Natural Science Foundation of China(No.81503616),Science and Technology Planning Project of Guangdong Province of China(No.2014A020221003),Project of Guangdong Province Administration of Traditional Chinese Medicine(No.20161218),Science and Technology Pro-ject of Shenzhen(No.JCYJ20160428105935612),Project of Health and Family Planning Commission of Shenzhen Municipality(No.201505019),Research Cultivation Fund of Hainan Medical University Supported Project(No.HY2018-34) （No.81503616）
