北京中医药大学学报2018，Vol.41Issue(2)：154-159,6.DOI:10.3969/j.issn.1006-2157.2018.02.010
电针对慢性应激抑郁模型大鼠海马及血清caspase-12表达的影响
Effects of electroacupuncture on the expression of caspase-12 in hippo-campus and serum of rats with chronic unpredictable mild stress
摘要
Abstract
Objective To observe the effects of electroacupuncture(EA)on the expression of central and peripheral cysteine aspartic acid protease-12 via JNK signal transduction pathway in rats with chronic stress depression and to explore anti-depression mechanism of electro-acupuncture.Methods SD rats were randomly divided into blank group,stress model group,acupuncture group,fluoxetine group,model+DMSO(dimethyl sulfoxide,soluble organic solvent)group, model+JNK pathway blocker SP600125 (hereinafter abbreviated as SP)group, acupuncture +SP group, and fluoxetine +SP group(n=9 in each group).The combination of chronic stress and solitary raising was used to establish chronic unpre-dictable mild stress model in seven groups except the control group.The investigated intervention was EA at"Neiguan(PC6)"and"Sanyinjiao(SP6)"points,20 min for each session,once a day,for 21 con-secutive days.The positive control group was treated with fluoxetine 5 mg/kg by oral gavage for 21 days. Open field test was used to observe the rats' behaviors.The expression of caspase-12 protein in hippo-campus and serum was measured by using ELISA.Results Before the experiment,there was no statistic difference in rat behaviors of all groups(P>0.05).At Day 21 of the experiment,the horizontal crossing lattice and frequency of vertical movements markedly decreased compared to the blank group(P <0.01).Compared with the model group,EA increased the horizontal crossing lattice(P<0.05).Com-pared with the blank group,the expression of caspase-12 protein in the model group increased significant-ly(P<0.01).Compared with the model group, the expression of caspase-12 protein in the model +DMSO group,fluoxetine group,fluoxetine+SP group,acupuncture group and SP group all decreased(P<0.05).Compared with the blank group,the expression of caspase-12 protein in the model group de-creased.Compared with the model group, the expression of caspase-12 protein in the model +DMSO group,model+SP group, fluoxetine group and acupuncture +SP group(P>0.05)decreased, while the expression of caspase-12 in fluoxetine+SP group and acupuncture group was higher than that in the control group,but all the differences were not statistically significant(P>0.05).Conclusion Acu-puncture treatment may reduce the expression of caspase-12 protein in the hippocampus of chronic stress rats by effectively inhibiting the JNK signal transduction pathway activity and reducing the apoptosis of hippocampal neuron,which contributes to its anti-depression mechanism.关键词
抑郁症/慢性应激/针刺/JNK信号通路/半胱氨酸天冬氨酸蛋白酶-12/大鼠Key words
depression/chronic stress/acupuncture/JNK signaling pathway/caspase-12/rats分类
医药卫生引用本文复制引用
田朝阳,邬继红,图娅,姜会梨,张旭辉,卢峻,俞秋云,梁星辰,邵淑君,向杜炼..电针对慢性应激抑郁模型大鼠海马及血清caspase-12表达的影响[J].北京中医药大学学报,2018,41(2):154-159,6.基金项目
国家自然科学基金资助项目(No.81173334)National Natural Science Foundation of China(No.81173334) （No.81173334）
