大豆科学2018，Vol.37Issue(1)：112-116,5.DOI:10.11861/j.issn.1000-9841.2018.01.0112
根瘤菌与烯效唑互作对大豆生长及固氮的影响
Effect of Uniconazole and Rhizobium Interaction on Growth and Nitrogen Fixation in Soybean
摘要
Abstract
Taking strains of Sinorhizobium fredii and Bradyrhizobium elkanii as the research materials,the concentration of uniconazole from 0.001 to 100 mg·L-1 were used to determine the influence of uniconazole interacting with those two strains.The results showed that the amount of S.fredii and B.elkanii was inhibited when the concentration of uniconazole was higher than 10 mg·L-1,but the amount of these two strains was promoted when the concentration of uniconazole was 1 mg· L-1.Soybean seed was presoaked by uniconazole with 1 mg·L-1and 30 mL Rhizobium liquid in logarithmic growth phase was mixed with soil per 2.5 kg.The enzyme activities of SOD,POD and CAT,the MDA level in soybean leaf and root,the ureide content in root were assayed in flowering-poding phase to reflect the effects of those two strains and uniconazole interaction on soybean.The results confirmed that those two strains and uniconazole interaction were effectively reduced the MDA level about 40％-60％ in soybean roots and leaves,the ureide content in soybean root were increased 5％-8.4％,however,the three enzyme activities were not significantly affected.The experimental results confirmed that Rhizobium interacting with uniconazole could increase nitrogen fixation ability of soybean and reduce MDA content.关键词
韦氏中华根瘤菌/埃尔坎慢生根瘤菌/烯效唑/大豆Key words
Sinorhizobium fredii/Bradyrhizobium elkanii/Uniconazole/Soybean分类
农业科技引用本文复制引用
张明会,王碧莹,李双,李可,陈阳,何斌,孙冬梅..根瘤菌与烯效唑互作对大豆生长及固氮的影响[J].大豆科学,2018,37(1):112-116,5.基金项目
保护地无公害蔬菜生产生物保障体系的开发应用(1254CGZH32) （1254CGZH32）
黑龙江八一农垦大学研究生创新科研项目(YJSCX2017-Y64). （YJSCX2017-Y64）
