分析化学2018，Vol.46Issue(3)：454-461,8.DOI:10.11895/j.issn.0253-3820.171175
分子印迹聚合物固相萃取-超高效液相色谱-串联质谱法检测水产品中11种氨基糖苷类药物残留
Determination of 11Kinds of Aminoglycosides in Aquatic Products by Ultra Performance Liquid Chromatography-Tandem Mass Spectrometry with Molecularly Imprinted Polymers Solid Phase Extraction
摘要
Abstract
An ultra performance liquid chromatography-tandem mass spectrometric (UPLC-MS/MS) method was developed for the determination of 11 kinds of aminoglycosides (AGs), including paromomycin, spectinomycin, tobramycin, gentamycin, kanamycin, hygromycin B, apramycin, streptomycin, dihydrostreptomycin,amikacin and neomycin in aquatic products. Samples were extracted by phosphate buffer solution, and purified on molecularly imprinted polymers (MIP) solid phase extraction column. After separated by Obelisc R chromatographic column, AGs were detected by UPLC-MS/MS. It showed a good linearity relationship in the AGs concentration range of 1.0-1000 ng/mL with the correlation coefficient R2>0.994. The limit of detection (LOD,S/N≥3) was ranged from 1.0 μg/kg to 10.0 μg/kg,and the limit of quantitation (LOQ,S/N≥10) was ranged from 2.0 μg/kg to 20.0 μg/kg. Besides, the average recoveries presented 78.4%-109.6% with the relative standard deviation (RSD, n=6) of 2.3%-14.9%. This method was successfully applied to the simultaneous determination of 11 kinds of AGs with high sensitivity in aquatic products.关键词
分子印迹聚合物固相萃取/超高效液相色谱-串联质谱/水产品/氨基糖苷类Key words
Molecularly imprinted polymers solid phase extraction/Ultra performance liquid chromatography-tandem mass spectrometric/Aquatic products/Aminoglycosides引用本文复制引用
黄原飞,娄晓祎,周哲,汪洋,孔聪,黄冬梅,蔡友琼,于慧娟..分子印迹聚合物固相萃取-超高效液相色谱-串联质谱法检测水产品中11种氨基糖苷类药物残留[J].分析化学,2018,46(3):454-461,8.基金项目
本文系上海市农业委员会资助项目(农沪科推字(2016)第1-4-1号)资助 This work was supported by the Shanghai Municipal Agricultural Commission(No.(2016)No.1-4-1). （农沪科推字(2016）
