中国妇幼健康研究2018，Vol.29Issue(2)：149-153,5.DOI:10.3969/j.issn.1673-5293.2018.02.005
应用蛋白质组学技术筛选抽动障碍患儿不同中医证型血清相关蛋白
Screening of serum related proteins of different TCM syndromes with tic disorders by proteomic techniques
摘要
Abstract
Objective To explore the differential expression of serum proteins in children with tic disorders(TD)and to screen the serum-specific differentially expressed proteins of TD children with different TCM syndromes.Methods Totally 60 children with TD(including 34 cases of syndrome of qi depression transforming into fire and 26 cases of spleen deficiency leading to phlegm syndrome)and 30 healthy children were enrolled in this study from April to September in 2017.Serum was separated from blood and the protein peaks were detected by Mass spectrometry based on magnetic particle.Results There was no significant difference in sex and age between TD group and healthy control group(χ2= 1.142,t= 0.112,both P< 0.05).There were 8 differentially expressed protein peaks between TD group and healthy control group,and their relative molecular mass(Mr)were 1 869.76, 6 443.34,6 642.05,863.13,2 175.98,2 191.84,2 277.19 and 2 293.11 with significant difference(P<0.001).Their AUC values were 0.793,0.831,0.861,0.779,0.778,0.783,0.781 and 0.754.There were 2 differentially expressed protein peaks between cases of syndrome of qi depression transforming into fire and cases of spleen deficiency leading to phlegm syndrome,and their relative molecular mass(Mr)were 873.73 and 6 642.04 with statistical significance(P<0.001).Their AUC values were 0.823 and 0.766.Conclusion There are differences in serum protein expressions between TD children and healthy controls.These differences may be helpful to further explore the pathogenesis of childhood TD or serve as biomarkers for clinical diagnosis.The differences between syndrome of qi depression transforming into fire and spleen deficiency leading to phlegm syndrome may be related to the micro-material of syndromes.It is of guiding significance to explore the biological basis of TCM syndrome and to establish diagnostic models.关键词
儿童抽动障碍/气郁化火证/脾虚痰聚证/蛋白组学/基质辅助激光解析电离飞行时间质谱Key words
tic disorders in children/syndrome of qi depression transforming into fire/spleen deficiency leading to phlegm syndrome/proteomics/MALDI-TOF-MS分类
医药卫生引用本文复制引用
王端,贺莉,张婕,赵小艳,熊晓藩,贺文香,张海清,陈艳妮..应用蛋白质组学技术筛选抽动障碍患儿不同中医证型血清相关蛋白[J].中国妇幼健康研究,2018,29(2):149-153,5.基金项目
国家自然科学基金面上资助项目(81371900) （81371900）
陕西省科技攻关资助项目(2013SF2-10) （2013SF2-10）
陕西省自然科学研究计划资助项目(2009JM4030). （2009JM4030）
