国际口腔医学杂志2018，Vol.45Issue(2)：170-176,7.DOI:10.7518/gjkq.2018.02.009
成骨细胞条件性FoxO1基因敲除糖尿病小鼠模型的建立
Establishment of diabetic mouse model with conditional knockout of FoxO1 in osteoblasts
摘要
Abstract
Objective This study aims to establish a diabetic mouse model with conditional knockout of FoxO1 in osteoblasts and preliminary explore the phenotypes. Methods Wild type (WT) and gene knockout mice were obtained according to the designed reproductive strategy. DNA was extracted from mouse tail and amplified by real-time polymerase chain reaction(RT-PCR).The mice phenotypes were also detected.The mRNA and protein levels of FoxO 1 in osteoblasts and other tissues were obtained and detected by RT-PCR and Western blot analysis. Mice weight and fasting blood glucose were measured.Insulin tolerance test(ITT)was conducted to assess the role of FoxO 1 in glucose metabolism.Results Diabetic mouse models with conditional knockout of FoxO1 in osteoblasts were successfully established. The body weight of diabetic mice was significantly lower than that of normal mice at 2 weeks. The fasting blood glucose in diabetic gene knockout mice was lower than that in diabetic WT mice.In addition,ITT showed that FoxO 1 knockout promoted insulin sensitivity in diabetic gene knockout mice compared with that in diabetic WT mice. Conclusion Diabetic mouse model with conditional knockout of FoxO1 in osteoblasts was successfully established. FoxO1 knockout ameliorated hyperglycaemia, which may be accounted for the increased insulin sensitivity in diabetic gene knockout mice.关键词
叉头转录因子/条件性基因敲除/糖尿病Key words
forkhead box protein O/conditional knock-out/diabetes mellitus分类
医药卫生引用本文复制引用
熊毅,宫苹,伍颖颖..成骨细胞条件性FoxO1基因敲除糖尿病小鼠模型的建立[J].国际口腔医学杂志,2018,45(2):170-176,7.基金项目
国家自然科学基金(81400543,81571008) （81400543,81571008）
四川大学优秀青年学者科研基金(A类)(2017SCU04A21)This study was supported by National Natural Science Foundation of China(81400543,81571008)and Outstanding Scholars Research Funding of Sichuan University(2017SCU04A21). （A类）
