广西植物2018，Vol.38Issue(2)：225-232,8.DOI:10.11931/guihaia.gxzw201704007
滴水珠珠芽发育过程研究
Bulbil development of Pinellia cordata
摘要
Abstract
Tuber of Pinellia cordata has been the extensively used as medicines in the practice of traditional Chinese medicine. Bulbil formation is the main propagation strategy of P. cordata in wild. There are two developmental stages of bulbils for each one individual: on the basis of leaf blade(upper bulbil)and on the lower part of petiole(lower bulbil). To reveal the developmental characteristics of bulbil formation in P. cordata,morphological and anatomical ob?servation were systematically used. Morphological observation showed that the whole development process of upper or lower bulbil could be divided three stages:no visible bulbil structure,primary bulbil formation under epidermis,growth and maturation of bulbil.Correspondingly,anatomical observation showed that the whole development process of upper or lower bulbil could be divided four stages:initial cell formation,bulbil promodia formation,differentiation of bulbil,and bulbil growth continuously to maturation. The initial cell of bulbil was derived or dedifferentiated from the parenchyma cell which was located in the adaxial side of young petiole.Initial cell formation was not visible.Continuously division of the initial parenchyma cell resulted in the formation of bulbil primodium,which was a group of undifferentiated cell.The primordium continuously growth and then differentiated to form the shoot apical meristem(SAM),which was the infant bulbil on the adaxial side. Bulbil promodium formation and early primodium differentiation could be visible with white dot structure on petiole.Subsequently,differentiated bulbil growth and lateral scale leaf differentiation continuously char?acterized the bulbil growth stage. Bulbil growth terminated with the leaf senescence. The average diameter of maturated upper bulbil on blade and lower bulbil on petiole were(2.79 ± 0.40)mm and(2.69 ± 0.36)mm, respectively. The maturated bulbils generally were gray and ellipsoidal,and shaped with various number of external scale leaf. The inner cells of maturated bulbil contained lots of nutrients(storage).The germination rate of bulbils was up to 75%in soil cul?ture. Morphological and anatomical observation results indicate that the development of bulbils in P.cordata is the same in P. ternata,and the development of bulbil in P. cordata is an effective reproduction strategy just like in P. ternata.关键词
珠芽/发育过程/形态特征/解剖结构/滴水珠Key words
bulbil/development process/morphological characteristis/anatomical structure/Pinellia cordata分类
农业科技引用本文复制引用
朱燕燕,罗睿,陈海丽,刘丹..滴水珠珠芽发育过程研究[J].广西植物,2018,38(2):225-232,8.基金项目
国家自然科学基金(31560081) （31560081）
贵州大学创新基金(2016006)[Supported by the Natural Science Foundation of China(31560081) （2016006）
Innovation Foundation of Graduate School of Guizhou University(2016006)]. （2016006）
