广西植物2018，Vol.38Issue(2)：241-249,9.DOI:10.11931/guihaia.gxzw201704024
不同耕作方式对当归根际土壤细菌群落多样性的影响
Effects of different cultivation methods on bacterial community diversity in rhizosphere soil of Angelica sinensis
摘要
Abstract
Angelica sinensis is a genuine regional drug in the Gansu Province of China,continuous cropping obstacles se?riously affect the yield and quality of angelica in recent years. In this paper,the rhizosphere soil was sampled from the fields of Angelica?soybean rotated, angelica continuous cultivations and wasteland in Weiyuan County of Gansu Province. We aimed to know the differences of community structure and diversity of bacteria population in the three sam?pling sites,and provide some useful advises for the reasonable planting of angelica.The method of 16s rRNA PCR?RFLP (polymerase chain reaction?restriction fragment length polymor?phism)was used for the comparative analysis of rhizo?sphere bacterial community structure and diversity. The total DNA was extracted from the soil microbes by CTAB?SDS method and 16S rRNA gene cloning library was established.The fingerprints were analyzed by restriction fragment length polymorphism(RFLP)by using the HaeⅢ,HhaⅠand HinfⅠrestriction enzymes,respectively. Then phylogenetic tree was set up according to the 16S rRNA sequences.After preliminary analysis,there was no significant difference in bacte?ria population quantity and diversity indices of rhizosphere soils among wasteland,rotated planted fields and continuous cultivation fields,but there was very obvious difference in community structure of bacteria among the three fields,espe?cially in rotated planted fields and continuous cultivation fields.The results showed that the dominant population of bac?teria was Proteobacteria in rotation fields and wasteland,while the dominant population of bacteria was Bacteroidetes in continuous cultivation fields of angelica.The strains of Sphingomonas that belonged to Proteobacteria,which were benefi?cial to the growth of crop, appeared only in the rotation fields and wasteland, and the Achromobacter that belonged to Bacteroidetes associated with the continuous cropping obstacle appeared only in the continuous cropping fields. Our re?sults suggested that the different ways of angelica cultivation play an important role in bacteria community structure. Meanwhile, the continuous cropping obstacle might be associated with the changes of bacterial community structure in rhizosphere soils of cropped angelica. In conclusion, we think that the rotation can effectively enhance the composition of the bacterial community structure,increase the beneficial bacterial population,improve the soil microen?vironment,and prevent the root rot of angelica.关键词
当归/连作障碍/根际微生物/16S rDNA/PCR⁃RFLPKey words
Angelica sinensis/continuous cropping obstacle/rhizosphere microbe/16S rDNA/PCR⁃RFLP分类
农业科技引用本文复制引用
霍庆迪,赵庆芳,马艳,李巧峡..不同耕作方式对当归根际土壤细菌群落多样性的影响[J].广西植物,2018,38(2):241-249,9.基金项目
国家自然科学基金(31560066)[Supported by National Natural Science Foundation of China(31560066)]. （31560066）
