工业微生物2018，Vol.48Issue(1)：8-13,6.DOI:10.3969/j.issn.1001-6678.2018.01.002
DCCD对植物乳杆菌H+-ATPase活性及基因表达水平的影响
Effects of DCCD on H +-ATPase activity and genes expression of Lactobacillus plantarum
摘要
Abstract
In this paper,the effects of H+-ATPase activity and genes expression by adding N,N'-dicyclohexyl carbodiimide (DCCD) were explored,and the mechanism of H +-ATPase regulation in Lactobacillus plantarum was studied.The growth ability,glucose consumption rate,lactic acid yield and H +-ATPase activity of wild-type ZUST and mutants ZUST-1,ZUST-2 were measured,respectively.Then,the real-time RT PCR was used to evaluate the relative quantification of the H +-ATPase genes expression.Results revealed that after adding DCCD,the growth ability,glucose consumption rate,lactic acid yield and H +-ATPase activity were lower than those without adding DCCD.All the H +-ATPase coding genes of ZUST and mutant ZUST-2 were higher than those in the exponential phase,which indicated that the expression of all genes was up-regulated due to the dual effects of fermentation acid stress and exogenous inhibitor DCCD.Compared with that without adding DCCD,the H +-ATPase expression levels of Lb.plantarum ZUST and mutant ZUST-2 were reduced or unchanged in the logarithmic phase,the activity of H +-ATPase was inhibited and the growth rate of the strains decreased.These results would pave the way for gaining further insights into the regulation mechanism of the H +-ATPase in Lb.plantarum under acid stress.关键词
植物乳杆菌/H+-ATPase/DCCD/荧光定量PCRKey words
Lactobacillus plantarum/H +-ATPase/DCCD/real-time RT PCR引用本文复制引用
谢东芳,张祥,花雨娇,杨俊兴,JIANG Ping,吕常江,方卉,黄俊..DCCD对植物乳杆菌H+-ATPase活性及基因表达水平的影响[J].工业微生物,2018,48(1):8-13,6.基金项目
国家自然科学基金(Nos.31240054),浙江省自然科学基金(Nos.LY16B060008,LQ18B060002),浙江科技学院研究生创新基金(No.2016YJSKC003). （Nos.31240054）
