湖南中医药大学学报2018，Vol.38Issue(2)：125-129,5.DOI:10.3969/j.issn.1674-070X.2018.02.003
食积胃肠积热动物模型的探索与评价
Establishment and Evaluation of Dyspeptic Model Animals with Accumulated Heat in Stomatch and Intestine
摘要
Abstract
Objective To establish and evaluate the dyspeptic animal model with accumulated heat in stomatch and intestine by administrating a high calorie diet. Methods The young SD rats were divided into the normal group and model group. Dyspeptic animal model with accumulated heat in stomatch and intestine was established by administrating a high calorie diet. The diagnosis and research of dyspeptic animal model with accumulated heat in stomatch and intestine were assessed by collecting the macroscopic characterization and detecting the metabolic index. Colon tissue was measured by HE staining, and the expression of NF-kB p65 in colon tissue was observed by immunohistochemistry to evaluate the intestinal inflammation in rats. Results Compared with the normal group, the rats in the model group were thinner, slower in weight gain, more aggressive, with less intake food (P<0.01), prolonged defecation time (P>0.05) and decreased defecation amount (P<0.01).Pathology showed that the colonic mucosa of rats became thinner and the number of goblet cells decreased. Local hyperemia, edema and fibrous hyperplasia were observed in the submucous lamina propria. The expression of NF- kappa B p65 in the colonic mucosa was diffusely distributed, with a deep brown and coarse grain, and a diffusion trend to the lamina propria. The model characterization was consistent with clinical diagnosis of infantile dyspepsia, which fitted the standard of dyspeptic animal model with accumulated heat in stomatch and intestine. Conclusion The method of administration with a high calorie diet can successfully establish the dyspeptic animal model with accumulated heat in stomatch and intestine, which is in accordance with the characteristics of TCM.关键词
食积/胃肠积热/高热量饲料/模型制备/模型评价Key words
dyspepsia/accumulated heat in stomatch and intestine/high heat fodder/model preparation/model evaluation分类
医药卫生引用本文复制引用
白辰,郑子安,徐竞男,万宇翔,王云辉,于河,刘铁钢,谷晓红..食积胃肠积热动物模型的探索与评价[J].湖南中医药大学学报,2018,38(2):125-129,5.基金项目
国家自然科学基金(81403328) （81403328）
北京中医药大学基本科研业务费自主选题(2017-JYB-XS-001). （2017-JYB-XS-001）
