湖南中医药大学学报2018，Vol.38Issue(2)：145-149,5.DOI:10.3969/j.issn.1674-070X.2018.02.007
黄芪解毒汤对乳腺癌细胞Jag1基因和CyclinD1蛋白表达影响的研究
Effect of Huangqi Jiedu Decoction on Expression of Jag1 and CyclinD1 in Breast Cancer Cells
摘要
Abstract
Objective To investigate the effect of Huangqi Jiedu decoction on the expression of Jag1 gene and CyclinD1 protein in MCF-7 cells of human breast cancer and to reveal its possible mechanism of inhibiting the recurrence and metastasis of breast cancer. Methods Drug-containing serum of Huangqi Jiedu decoction group, doxorubicin group, Shenyi capsule group and normal saline serum group were built by using the method of gavage in SPF SD rats. The optimal concentration of cell inhibition routine culture of cells was detected by MTT. The breast cancer was intervened with 20% drug containing serum. The expression of JAG1 protein was detected by real-time quantitative PCR and CyclinD1 protein was determined by Western-blot. Results Compared with the normal saline group, the expression of Jag1 and CyclinD1 protein in the other serum groups significantly decreased (P<0.01). The inhibitory effect of the adriamycin serum group was the strongest (P<0.01). There was no significant difference between the serum group of Huangqi Jiedu decoction group and Shenyi capsule group (P> 0.05). Conclusion Huangqi Jiedu decoction could significantly prohibit the expression of Jag1 and CyclinD1, and provide the experimental evidence for Huangqi Jiedu Decoction in the treatment of breast cancer after surgery.关键词
MCF-7/Jag1/CyclinD1/黄芪解毒汤Key words
MCF-7/Jag1/CyclinD1/Huangqi Jiedu decoction分类
医药卫生引用本文复制引用
熊家青,吴世婷,李逵,刘丽芳..黄芪解毒汤对乳腺癌细胞Jag1基因和CyclinD1蛋白表达影响的研究[J].湖南中医药大学学报,2018,38(2):145-149,5.基金项目
湖南省科技厅项目(2012SK3137),湖南省教育厅项目(13A071). （2012SK3137）
