临床口腔医学杂志2018，Vol.34Issue(2)：67-70,4.DOI:10.3969/j.issn.1003-1634.2018.02.002
YAP在牵张力介导的人牙周膜细胞成骨分化中的作用
Role of YAP in tension force-induced osteogenesis of human periondontal ligament cells
摘要
Abstract
Objective:To investigate the role of Yes-associated protein(YAP)in tension force-induced osteoblast differentiation in human periodontal ligament cells.Methods:HPDLCs were acquired by enzyme digestion method.Firstly,the cells were stretched and subjected to immunofluorescence assay to detect the changes of YAP location in hPDLCs,after being stretched for 1 d and 3 d,the qRT-PCR and alkaline phosphatase staining assay were performed respectively to detect the ex-pression of osteogenesis-related genes(ALP,DLX5,RUNX2)and ALP activity.Secondly,hPDLCs were transfected with YAP SiRNA or negative control SiRNA and stretched for 1 d and 3 d,osteogenesis-related genes and alkaline phosphatase staining assay were used to analyse the influence of YAP on tension force-induced osteogenic differentiation of hPDLCs.Results:Nu-clear localization of YAP was significantly facilitated by tension force,and compared with control,stretched hPDLCs showed remarkable increase in osteogenesis-related genes and ALP activity.After transfection with YAP SiRNA,the expression of os-teogenesis-related genes and ALP activity were significantly attenuated in stretched hPDLCs,compared with those transfected with negative control SiRNA.Conclusion:YAP knockdown leads to inhibition of tension force-induced osteogenesis in hP-DLCs,and it may serve as a new therapeutic target for orthodontic treatment.关键词
人牙周膜细胞/成骨分化/Yes相关蛋白/牵张力Key words
Human periodontal ligament cells/Osteogenic differentiation/Yes-associated protein/Tension force分类
医药卫生引用本文复制引用
杨洋,韩光丽..YAP在牵张力介导的人牙周膜细胞成骨分化中的作用[J].临床口腔医学杂志,2018,34(2):67-70,4.基金项目
国家自然科学基金面上项目(81371169,81771111) （81371169,81771111）
