临床口腔医学杂志2018，Vol.34Issue(2)：77-79,3.DOI:10.3969/j.issn.1003-1634.2018.02.005
脂多糖对人牙周韧带细胞中MT1-MMP表达的影响
Effects of lipopolysaccharide on MT1-MMP expression in human periodontal ligament cells
摘要
Abstract
Objective:To investigate the effects of lipopolysaccharide in graded concentration and stimulating time on MT1-MMP expression in human periodontal ligament cells.Methods:Cultured hPDLCs were stimulated with lipopolysaccha-ride in concentration and time gradient respectively.Western Blot and quantitative real-time PCR were employed to determine MT1-MMP expressions at protein and gene level.Results:LPS stimulation reduced the expressions of MT1-MMP protein and mRNA,and the regulatory effects of LPS exhibited a certain concentration and time dependence(P<0.05).Conclusion:MT1-MMP expressions were significantly enhanced in LPS-stimulated hPDLCs,MT1-MMP may be involved in inflammatory reactions of periodontal tissues.关键词
脂多糖/牙周韧带细胞/MT1-MMPKey words
Lipopolysaccharide(LPS)/periodontal ligament cell/MT1-MMP分类
医药卫生引用本文复制引用
刘念,曹颖光,朱光勋..脂多糖对人牙周韧带细胞中MT1-MMP表达的影响[J].临床口腔医学杂志,2018,34(2):77-79,3.基金项目
国家自然科学基金项目(81300883) （81300883）
武汉市创新人才开发资金(2014年度) （2014年度）
同济医院回国人员启动基金(2016年度) （2016年度）
