临床与实验病理学杂志2017，Vol.33Issue(11)：1199-1202,4.DOI:10.13315/j.cnki.cjcep.2017.11.005
沉默CD98hc表达对乳腺癌细胞MDA-MB-231增殖、侵袭的影响
Effect of silencing CD98hc expression on MDA-MB-231 breast cancer cells proliferation and invasion
摘要
Abstract
Purpose To investigate the effect of silencing of CD98hc on proliferation,migration and invasion of MDA-MB-231 breast cancer cells.Methods RNA interfere technology was used to silence CD98hc in MDA-MB-231 cells.MDA-MB-231 cells (CD98hc-shRNA) with stable low expression of CD98hc and Vector control was obtained.Western blot and qRT-PCR were used to verify the down-regulation of CD98hc.MTT assay was used to test the cell proliferation.Transwell migration and invasion experiment were used to assay cell migration and invasion.Results The expression levels of CD98hc was down-regulated by CD98hc-shRNA in the MDA-MB-231 breast cancer cells.Compared to that of blank cells (0.706 ± 0.013),vector control (0.724 ± 0.018),the cell proliferation potency of CD98hc-shRNA cells (0.580-0.035) was significantly inhibited (P ＜ 0.05),and the cell migration and invasion potency also were inhibited (P ＜ 0.05),there is on significant different between blank cells and vector control (P ＜ 0.05).Conclusion CD98hc might be involved in the regulation of breast cancer cell biological behaviors.关键词
乳腺肿瘤/CD98hc/细胞增殖/细胞侵袭Key words
breast neoplasm/CD98hc/cell proliferation/cell invasion分类
医药卫生引用本文复制引用
牟浪,吴正升,吴强..沉默CD98hc表达对乳腺癌细胞MDA-MB-231增殖、侵袭的影响[J].临床与实验病理学杂志,2017,33(11):1199-1202,4.基金项目
国家自然科学基金(81372476、81472493) （81372476、81472493）
