临床与实验病理学杂志2017，Vol.33Issue(11)：1203-1208,6.DOI:10.13315/j.cnki.cjcep.2017.11.006
CXCL12/CXCR4/MMP-2信号通路对三阴型乳腺癌生物学行为的影响
Effect of CXCL12/CXCR4/MMP-2 signaling pathway on biological behavior in triple negative breast cancer
摘要
Abstract
Purpose To investigate the effect of CXCL12/CXCR4 and MMP-2 on the biological behavior of triple-negative breast cancer.Method CXCR4-siRNA and pcDNA3.1-CXCR4were transient transfected of in MDA-MB-231 cell line by liposome,RNA and protein were extracted.The mRNA and protein expressions of CXCR4,CXCL12 and MMP-2 were detected by RT-PCR and Western blot respectively.And the migration and proliferation of the transfected cells were detected by Wound-healing assay and MTF assay.Result The experiment of RT-PCR and Westen blot demonstrated that CXCR4 mRNA and protein level expression declines after the expression CXCR4 was down-regulated (P ＜ 0.01),CXCL12 mRNA and protein level expression increased and MMP-2 mRNA and protein level expression increased after the expression CXCR4 was down-regulated (P＜0.01),MTT assay and Wound-healing assay results showed that the proliferation ability of 72 h cells was decreased,and the wound healing was slow.The experiment of RT-PCR and Westen blot demonstrated that CXCR4 mRNA and protein level expression increased after the expression CXCR4 was up-regulated (P ＜0.01).The CXCL12 mRNA and protein level expression declined and MMP-2 mRNA and protein level expression increased after the expression CXCR4 was up-regulated (P ＜0.01),MTT assay and wound-healing assay results showed that the proliferation ability of 72 h cells was increased,and the wound healing was accelerated.Conclusion CXCL12/CXCR4/ MMP-2 signaling pathway is activated in the triple-negative breast cancer MDA-MB-231 cells,and the inhibition of CXCR4 can reverse the proliferation and migration of malignant biological process.关键词
乳腺肿瘤/三阴型乳腺癌/CXCR4/CXCL12/MMP-2Key words
breast neoplasm/triple negative breast cancer/CXCR4/CXCL12/MMP-2分类
医药卫生引用本文复制引用
焦娟,宋艳艳,刘霞,刘倩,冯志寅,苏丽萍,何鼎盛,张巍..CXCL12/CXCR4/MMP-2信号通路对三阴型乳腺癌生物学行为的影响[J].临床与实验病理学杂志,2017,33(11):1203-1208,6.基金项目
新疆维吾尔自治区自然科学基金(2014211C049) （2014211C049）
