临床与实验病理学杂志2017，Vol.33Issue(11)：1219-1224,6.DOI:10.13315/j.cnki.cjcep.2017.11.009
自噬相关基因Beclin1和LC3在卵巢浆液性癌中的表达及临床意义
Expression of autophagy-related genes Beclin1 and LC3 in ovarian serous carcinoma and their clinical significance
摘要
Abstract
Purpose To detect the expression of autophagy-related genes Beclin1 and microtubule-associated protein1 light chain3 (LC3) in ovarian serous carcinoma (OSC) and to analyze the correlations with clinical pathological characteristics and prognosis of patients with OSC.Methods Immunohistochemical staining of MaxVision two-step was performed to detect the expression of Beclin1 and LC3 in the samples from 63 OSC patients and 20 with benign ovarian serous cystadenomas.The relation between Beclin1 and LC3 with the factors influencing the prognosis of OSC was investgated.The expression levels of mRNA and proteins in 10 fresh OSC samples and their corresponding adjacent noncancerous tissues were examined by RT-PCR and Western blot.Results The positive percentage of Beclin1 and LC3 protein in the tissues of OSC was 36.51％ and 33.33％,which was significantly lower than that of 65.00％ and 60.00％ in serous cystadenomas (P =0.025,P =0.034).The expression of Beclin1 in OSC was significantly correlated with clinical FIGO stage and pathological grade (P =0.001,P =0.001),but not associated with age,site,tumor size and lymph node metastasis (P ＞ 0.05).The expression of LC3 protein in OSC was significantly with clinical FIGO stage and lymph node metastasis (P =0.013,P =0.041),but not associated with age,site,tumor size and pathological grade (P ＞ 0.05).There was a positive correlation between Beclin1 and LC3 in OSC (rs =0.373,P =0.03).The levels of Beclin1 and LC3 mRNA (0.581-±0.091,0.650 ±0.090) in 10 fresh OSC were significantly lower than in their adjacent noncancerous tissues (t=8.083,t =6.614,P =0.016,P =0.022).The levels of Beclin1 and LC3 protein in 10 fresh OSCs were significantly lower than in their adjacent noncancerous tissues (P ＜ 0.05).Kaplan-Meier survival analyses revealed that the expression of Beclin1 and LC3 were associated with the patients prognosis (P =0.028 3,P =0.018 5).Conclusion Expression of Beclin1 and LC3 protein is down-regulated in the tissues of OSC which lead to decrease of function of autophagy.The decrease of Beclin1 and LC3 may be associated with the development and prognosis of OSC.关键词
卵巢肿瘤/卵巢浆液性癌/Beclin1/LC3/自噬/预后Key words
ovarian neoplasm/ovarian serous carcinoma/Beclin1/LC3/autophagy/prognosis分类
医药卫生引用本文复制引用
晋龙,眭玉霞,孙阳,陈小岩,陈志忠,陈灵锋,李艳辉,王丽萍..自噬相关基因Beclin1和LC3在卵巢浆液性癌中的表达及临床意义[J].临床与实验病理学杂志,2017,33(11):1219-1224,6.基金项目
福建省自然科学基金(2015J01424)、福建省立医院院内优秀青年基金(2014074) （2015J01424）
