生态与农村环境学报2018，Vol.34Issue(2)：140-145,6.DOI:10.11934/j.issn.1673-4831.2018.02.006
花粉源大小及花粉竞争对抗病毒转基因小麦基因漂移的影响
Effects of Size of Pollen Source and Competition of Pollens on Gene Flow of Virus-Resistant Transgenic Wheat
摘要
Abstract
Studying gene flow of transgenic wheat helps establish a corresponding strategy to prevent transgene escape and germplasm contamination between compatible wheat genotypes. An experiment was carried out using transgenic wheat with resistance to wheat yellow mosaic virus(WYMV)as pollen donor,and Yangmai 158 and dwarf male-sterile wheat as pol-len receptors,and two plots of the transgenic wheat,different in size(100 and 400 m2)and laid out in parallel as pollen sources. Gene flow frequency was monitored at different intervals from the pollen source to investigate effects of size of the pollen source and competition between pollens on gene flow of the transgenic wheat. Results show that size of the pollen source did not significantly affect transgene flow frequency, but variety of the two pollen receptors did. The male-sterile dwarf wheat was found to be significantly higher in transgene flow frequency at distance intervals of 0 and 2 m from the pol-len source. The gene flow frequency of transgenic wheat declined dramatically with increasing distance from the source and was reduced to 0 over the test distance of 5 m. All the findings indicate that pollen competition may be a major factor influ-encing transgene flow and isolation by distance is one of the most effective methods to control pollen-mediated gene flow in wheat.关键词
转基因漂移/转基因小麦/花粉竞争/异交率/距离隔离Key words
transgene flow/transgenic wheat/pollen competition/outcrossing rate/isolation by distance分类
资源环境引用本文复制引用
董姗姗,石雪,于赐刚,张振华,陈明,刘燕..花粉源大小及花粉竞争对抗病毒转基因小麦基因漂移的影响[J].生态与农村环境学报,2018,34(2):140-145,6.基金项目
转基因生物新品种培育科技重大专项(2016ZX08011-003,2016ZX08012-005) （2016ZX08011-003,2016ZX08012-005）
