中南医学科学杂志2018，Vol.46Issue(1)：23-27,5.DOI:10.15972/j.cnki.43-1509/r.2018.01.006
二烯丙基二硫对人白血病细胞增殖和钙网蛋白表达的影响
Effect of Diallyl disulfide on cell proliferation and calreticulin expression in human leukemia HL-60 cells
摘要
Abstract
To observe the effect of Diallyl disulfide (DADS) on the cell proliferation and expression of calreticulin (CRT) in human leukemia HL-60 cells.HL-60 cells were incubated with DADS (1.25mg/L) for 24,48 and 72 h.Expres-sion of CRT was inhibited by siRNA.Cell viability was tested by MTT assay.Expression of CRT was test by real-time quanti-tative PCR and Western bolt.Compared with the control group,the proliferation of HL-60 cells was significantly decreased in time dependent manner after treatment with DADS for 24,48 and 72 h.Compared with the control group,Expression of CRT was significantly decreased by transient transfection of siRNA of CRT.There was a synergistic effect between DADS and siR-NA of CRT on expression of CRT.Compared with the control group,the proliferation of HL-60 cells was significantly de-creased by siRNA of CRT.There was a synergistic effect between DADS and siRNA of CRT on the proliferation of HL-60 cells.DADS inhibits cell proliferation in human leukemia HL-60 cells,which mechanism may be related with down-regulation of CRT expression.关键词
二烯丙基二硫/钙网蛋白/人白血病HL-60细胞/细胞增殖Key words
Diallyl disulfide/calreticulin/human leukemia HL-60 cells/cell proliferation分类
医药卫生引用本文复制引用
陈歆,黄磊,王学锋,尹艳华,熊炜,邓雪冰,阳海华,苏琦,伍世葵..二烯丙基二硫对人白血病细胞增殖和钙网蛋白表达的影响[J].中南医学科学杂志,2018,46(1):23-27,5.基金项目
湖南省卫生与计划委员会课题(C2014-20). （C2014-20）
