中南医学科学杂志2018，Vol.46Issue(1)：28-31,4.DOI:10.15972/j.cnki.43-1509/r.2018.01.007
姜黄素调节AKT/PI3K活性抑制人膀胱癌T24细胞的增殖和迁移
Curcumin inhibits proliferation and migration of human bladder cancer T24 cell line by modulating AKT/PI3K signaling pathway
肖红照 1许韩峰 2贺书云 1庞高明 2郑云 2曹友汉2
作者信息
- 1. 南华大学附属第一医院泌尿外科,湖南 衡阳421001
- 2. 湘潭市湘潭县人民医院泌尿外科
- 折叠
摘要
Abstract
To study the effect of curcumin on the proliferation and invasion of bladder cancer T24 cells and its mech-anism.The bladder cancer T24 cells were cultured and treated with different concentrations of Curcumin.The CCK8 assay was used to detect the inhibitory growth rate of the curcumin on T24 cells.The transwell matrix penetration assay was used to detect the cell migration capabilities.The expressions of MMP2(matrix metalloproteinase),MMP9,AKT,p-AKT,PI3K and p-PI3K protein were analyzed by Western blotting.CCK8 results show that curcumin can inhibit the proliferation of T24 cells in terms of concentration and time.Transwell results demonstrate that the intervention of curcumin can significantly reduce the migration of bladder cancer T24 cells.Curcumin intervention can reduce the expressions of p-AKT,p-PI3K,MMP2 and MMP9 in bladder cancer T24 cells in a dose-dependent manner.The higher the curcumin concentration was used,the lower expression of p-AKT,p-PI3K,MMP2 and MMP9 were observed in T24 cells.The results showed curcumin may down-regulate the expression of MMP2 and MMP9 by suppressing the AKT/PI3K signaling pathway,thereby inhibiting the prolif-eration and migration of bladder cancer T24 cell.关键词
膀胱癌/姜黄素/AKT/PI3K途径/金属基质酶Key words
bladder cancer/curcumin/AKT/PI3K pathway/matrix metalloproteinase分类
医药卫生引用本文复制引用
肖红照,许韩峰,贺书云,庞高明,郑云,曹友汉..姜黄素调节AKT/PI3K活性抑制人膀胱癌T24细胞的增殖和迁移[J].中南医学科学杂志,2018,46(1):28-31,4.
