中南医学科学杂志2018，Vol.46Issue(1)：32-36,5.DOI:10.15972/j.cnki.43-1509/r.2018.01.008
喉癌相关基因的生物信息学分析及真核表达载体的构建
Bioinformatics analysis and construction of eukaryotic expression vector of Laryngeal cancer related gene protein
摘要
Abstract
Laryngeal cancer related gene (LCRG1) is a candidate tumor suppressor gene of laryngeal carcinoma.At present,there are few monoclonal antibodies that can be used for immunohistochemistry of clinicopathological specimens. This study analyzed the secondary structure and epitope of LCRG1 protein throμgh bioinformatics,and obtained that the most likely epitope region is 9-22,41-52,91-100,103-110,125-134,145-148,151-163,166-177,187-192,209-225,235-242,251-259,273-282 et al.The LCRG1 fragment was amplified by PCR,and 867bp was obtained.The fragment was inserted into eukaryotic vector V152H,to construct eukaryotic expression plasmid V152H-LCRG1.The plasmid was trans-fected into human embryonic kidney HEK293 cell.After successful expression by SDS-PAGE verified,the plasmid was puri-fied by Ni2+2NTA column chromatography to obtain protein with purity>95%,which laid a theoretical foundation for screening monoclonal antibodies.关键词
喉癌相关基因/喉癌/抗原表位/重组蛋白Key words
Laryngeal carcinoma related gene/Laryngeal carcinoma/epitope/recombinant protein分类
医药卫生引用本文复制引用
杨淑梅,谢海龙,李晓杰..喉癌相关基因的生物信息学分析及真核表达载体的构建[J].中南医学科学杂志,2018,46(1):32-36,5.基金项目
郴州市科技局资助项目(CZ2013082). （CZ2013082）
