同济大学学报（医学版）2017，Vol.38Issue(5)：7-12,6.DOI:10.16118/j.1008-0392.2017.05.002
miR-29b调节TET蛋白对脂肪来源的间充质干细胞成骨分化的影响
MiR-29b affects osteogenic differentiation of adipocyte-derived mesenchymal stem cells by modulating TET protein
摘要
Abstract
Objective To investigate the mechanism of miRNA-29b regulating the function of DNA demethylase TET protein in stem cells during directional differentiation and early embryonic development.Methods In vitro validation of target miRNAs was performed by bioinformatics methods combined with double luciferase reporter assay and in vivo validation was performed by fertilized egg microinjection,and then the influence of target miRNAs on Tets genes in directional differentiation of adipose-derived stromal cells (ASCs) was identified.Results The three mouse Tets genes were the target genes of miR-29abc,and the miR-29b showed the highest inhibition efficiency(P ＜ 0.05).Mutation of miR-29b by using CRISPR/Cas9 technology enhanced the expression of Tets and significantly promoted the efficiency of osteogenesis differentiation of ASCs(P ＜ 0.05).Conclusion MiR-29b can down-regulate the expression of Tets genes,and down-regulation of miR-29b can significantly promote the differentiation of adipocyte-derived mesenchymal stem cells into osteoblasts.关键词
miR-29b/Tets/间充质干细胞/成骨细胞分化Key words
miR-29b/Tets/adipose-derived stromal cells/osteogenic differentiation分类
生物科学引用本文复制引用
秦兵莲,沈君颜,陈嘉瑜,刘海亮..miR-29b调节TET蛋白对脂肪来源的间充质干细胞成骨分化的影响[J].同济大学学报（医学版）,2017,38(5):7-12,6.基金项目
国家重点基础研究发展(973)计划(2012CB966903) （973）
国家自然科学基金(31671539) （31671539）
