同济大学学报（医学版）2017，Vol.38Issue(5)：18-23,6.DOI:10.16118/j.1008-0392.2017.05.004
KLF2过表达及敲减胃癌稳转细胞株的建立
Establishment of gastric cancer cell lines with over-expressed KLF2 and knockdown KLF2
摘要
Abstract
Objective To establish gastric cancer cell line with over-expressed KLF2 and knockdown KLF2.Methods The recombinant plasmid GV358-KLF2 was constructed by cloning human KLF2 gene into AgeI/AgeI enzyme-digested GV358 vector,then the GV358-KLF2 plasmid was transfected into 293T cells to pack lentivirus.Three pairs of synthesized shRNAs were inserted into BamHI and EcoRI enzyme-digested PHBLV-U6-ZSGreen-Puro vector,and packed into lentivirus.The expression of KLF2 was detected by RT-PCR and Western blot.Results Recombinant plasmids GV358-KLF2 and KLF2-shRNA were successfully transfected into BGC823 and MGC803 respectively,and expressed stably.The results of RT-PCR and Western blotting showed that the expression of KLF2 was increased in KLF2-overexpressed BGC823 cells (P ＜ 0.05),and decreased in KLF2-knockdown MGC803 cells (P ＜ 0.05).Conclusion KLF2-overexpression BGC823 cell line and KLF2-knockdown MGC803 cell line have been successfully established,which may be used for the study of KLF2 function.关键词
KLF2基因/稳转株/敲减稳转/BGC823细胞/MGC803细胞Key words
Krüppel-like factor 2/overexpression cell line/knockdown cell line/BGC823 cell/MGC803 cell分类
医药卫生引用本文复制引用
王春梅,陈金联..KLF2过表达及敲减胃癌稳转细胞株的建立[J].同济大学学报（医学版）,2017,38(5):18-23,6.基金项目
上海市自然科学基金(16ZR1429100) （16ZR1429100）
