同济大学学报（医学版）2017，Vol.38Issue(5)：24-27,4.DOI:10.16118/j.1008-0392.2017.05.005
MRL/lpr小鼠干眼模型的实验研究
MRL/lpr mice as a dry eye mouse model
摘要
Abstract
Objective To examine whether the MRL/lpr mice with autoimmune lacrimal gland disease can be used as a dry eye mouse model.Methods Thirty 8-week-old female MRL/lpr mice (n =15) and BLAB/C mice (n =15) were used as experimental group and the control group,respectively.The animals were observed at 8,12,16 and 20 weeks.Cotton thread test was performed and the tear volume was measured.H-E staining was performed for lacrimal gland tissues and the infiltration of lymphocytes was analyzed.The expression of TNF-α and IL-1β in lacrimal gland was detected by Western blot.Results Tear production was significantly decreased in the experimental group than that in the control group (P ＜0.05),especially at 16 and 20 weeks.HE staining showed more infiltrating lymphocytes in the experimental group in comparison with the control group.According to Western blot,the expression of inflammatory factors TNF-α and IL-1β in lacrimal gland was increased in experimental group (P ＜ 0.05).With the progression of the disease in MRL/lpr mice,the infiltrating lymphocytes and the TNF-α and IL-1β levels increased gradually (P ＜ 0.05).Conclusion MRL/Ipr mice present the similar manifestations of human dry eye and the physiological and pathological changes in lacrimal gland,indicating that MRL/lpr mice can be used as a stable and feasible dry eye model.关键词
MRL/lpr小鼠/干眼/动物模型/炎症因子/泪腺Key words
MRL/lpr/dry eye/animal model/inflammation cytokine/lacrimal gland分类
医药卫生引用本文复制引用
刘宇,盛敏杰,李冰,陈丽,陆佳骏,陈轶卉..MRL/lpr小鼠干眼模型的实验研究[J].同济大学学报（医学版）,2017,38(5):24-27,4.基金项目
国家自然科学基金青年项目(81400373) （81400373）
同济大学附属杨浦医院课题(Se1201521) （Se1201521）
