同济大学学报（医学版）2017，Vol.38Issue(5)：33-36,40,5.DOI:10.16118/j.1008-0392.2017.05.007
原发性大隐静脉曲张中lncRNAs的差异表达
Aberrantly expressed lncRNAs in primary varicose great saphenous veins
摘要
Abstract
Objective To identify aberrantly expressed lncRNAs in great saphenous vein (GSV)varicosity and their potential functions.Methods Six pairs of samples of varicose vein and normal vein were used for lncRNAs expression microarray and 32 samples were used for quantitative qRT-PCR validation.Hierarchical clustering was performed.Gene ontology analysis and pathway analysis were performed to identify mRNA pathways.Results Total 557 lncRNAs and 980 mRNAs that differed between the varicose veins and normal veins were identified in six pairs of samples.These lncRNAs were sub-grouped and mRNAs expressed at different levels were clustered into several pathways with six focused on metabolic pathways,qRT-PCR validated six lncRNAs were consistent with the results of microarray.A coding-non-coding gene co-expression network revealed that four of these six lncRNAs might be correlated with 11 mRNAs and pathway analysis revealed that they might be correlated with another 8 mRNAs associated with metabolic pathways.Conclusion Aberrantly expressed lncRNAs for GSV varicosity have been systematically screened and validated and their functions predicted.These findings provide novel insight into the physiology of lncRNAs and the pathogenesis of varicose veins for further investigation.These aberrantly expressed lncRNAs may serve as new therapeutic targets for varicose veins.关键词
大隐静脉曲张/长链非编码RNA/基因芯片Key words
varicosis of great saphenous veins/long non-coding RNAs/gene microarray分类
医药卫生引用本文复制引用
陈国君,葛进,周斌,王翔,洪毅,沈瑶..原发性大隐静脉曲张中lncRNAs的差异表达[J].同济大学学报（医学版）,2017,38(5):33-36,40,5.基金项目
同济大学青年优秀人才培养行动计划(2014KJ081) （2014KJ081）
