上海针灸杂志2017，Vol.36Issue(11)：1367-1371,5.DOI:10.13460/j.issn.1005-0957.2017.11.1367
电针对高血压前期Dahl大鼠肾脏CaM/NO通路的影响
Effect of Electroacupuncture on Renal CaM/NO Pathway in Dahl Rats with Prehypertension
摘要
Abstract
Objective To investigate the effect of electroacupuncture on renal CaM-eNOS-NO signal pathway in Dahl salt-sensitive rats with prehypertension.Methods Thirty 7-week-old male Dahl salt-sensitive rats (DS) were allocated,using a random number table,to model,acupuncture and non-meridian-acupoint groups.Ten 7-week-old male Dahl salt-resistant rats (DR) were used as a blank group.The rats were fed in a SPF animal experiment center.An 8％ NaC1 high salt diet was provided after 1-week adaptive feeding.During the period,rat blood pressure was monitored using an intelligent noninvasive sphygmomanometer.A rat model of prehypertension was successfully made if rat blood pressure rose to 120～ 139/80～89 mmHg after 12 days.At that time,a high salt diet was stopped and a normal diet was provided.The blank and model groups received 15-minute regular fixation and no treatment.The acupuncture group received electroacupuncture at bilateral Quchi and Zusanli.The non-meridianacupoint group received electroacupuncture at fixed control points selected between 10 and 15 mm above bilateral iliac crests 20 mm lateral to the midline.Treatment was given once daily,6 times a week,for five consecutive weeks.The left rat kidney was taken.CaM and eNOS mRNA expressions were determined by quantitative fluorescence PCR.eNOS was quantified and located by western blot and immunohistochemical method.NO content was measured by ELISA.Results As compared with the blank group,CaM and eNOS mRNA expressions,eNOS protein content and NO content decreased significantly in the model group (all P＜0.05).As compared with the model group,CaM and eNOS mRNA expressions,eNOS protein content and NO content increased significantly in the acupuncture group (all P＜0.05).As compared with the model group,CaM expression increased significantly (P＜0.05) but eNOS mRNA expression and NO content had no statistically significant differences (P＞0.05) in the non-meridian-acupoint group.Conclusion The renoprotective effect of electroacupuncture at Quchi and Zusanli may be related to upregulating renal CaM-eNOS-NO signal pathway in DS rats with prehypertension.关键词
针刺疗法/电针/高血压前期/Dahl盐敏感大鼠/eNOS/一氧化氮/穴,曲池/穴,足三里Key words
Acupuncture therapy/Electroacupuncture/Prehypertension/Dahl salt-sensitive rats/eNOS/Nitric oxide/Point, Quchi/Point, Zusanli分类
医药卫生引用本文复制引用
王建波,曲怡,张立德..电针对高血压前期Dahl大鼠肾脏CaM/NO通路的影响[J].上海针灸杂志,2017,36(11):1367-1371,5.基金项目
辽宁省教育厅创新团队项目(LT2014021) （LT2014021）
辽宁省自然科学基金(20170540593) （20170540593）
