世界中医药2017，Vol.12Issue(10)：2419-2422,4.DOI:10.3969/j.issn.1673-7202.2017.10.036
益气活血组方对大鼠缺血心肌血管新生作用及对血管内皮生长因子和碱性成纤维生长因子的影响
Effect of Yiqi huoxue Decoction on Angiogenesis and Expression of VEGF and bFGF in the Ischemic Myocardium of Rats
摘要
Abstract
Objective:To investigate the effects of Yiqi Huoxue Decoction on the angiogenesis and the protein expression of vascular endothelial growth factor (VEGF) and basic fibroblast growth factor(bFGF) in the ischemic myocardium of rats with acute myocardial infarction.Methods:After the animal model of acute myocardial infarction (AMI) in rats is established,40 males SD rats were divided into 4 groups by random number table:model group,sham operation group,Shexiangbaoxin group and Yiqi Huoxue Formula group.The microvessel count(MVC),microvessel density (MVD) and expression of VEGF and bFGF in the ischemic myocardium were detected by immunohistochemistry.The change levels of VEGF and bFGF protein in the ischemic myocardium were detected by Western blot.Results:Yiqi Huoxue Formula group and Shexiangbaoxin group were compared with the model group,the infarcted myocardium edge area of microvessel count and microvessel density were significantly higher than that of the latter(P ＜0.01).The expression of VEGF and bFGF about Yiqi Huoxue Formula group and Shexiangbaoxin group and its protein expression level was significantly increased compared with the other groups(P ＜ 0.01).Conclusion:Yiqi Huoxue Formula group can significantly promote local angiogenesis in ischemic myocardium of rats after myocardial infarction,upregulation of myocardial VEGF and bFGF protein may be one of important mechanisms.关键词
益气活血/血管新生/血管内皮生长因子/碱性成纤维生长因子Key words
Yiqi Huoxue/Angiogenesis/VEGF/bFGF分类
医药卫生引用本文复制引用
胡国恒,朱荣华,肖青,边冠积..益气活血组方对大鼠缺血心肌血管新生作用及对血管内皮生长因子和碱性成纤维生长因子的影响[J].世界中医药,2017,12(10):2419-2422,4.基金项目
湖南省科技厅计划项目(2015SK20161 （）
2015SK2016) （）
