水生生物学报2017，Vol.41Issue(6)：1186-1192,7.DOI:10.7541/2017.147
斑节对虾α-淀粉酶基因的克隆及其表达分析
CLONING AND EXPRESSION ANALYSIS OF ALPHA AMYLASE CDNA OF PENAEUS MONODON
摘要
Abstract
In order to study the structure and biological function of the alpha-amylase gene in Penaeus monodon,the full-length cDNA sequence of α-Amylase from Penaeus monodon (PmAmy) was obtained by high throughput transcriptome sequencing and RACE.The PmAmy cDNA included an open reading frame of 2175 bp encoding a polypeptide of 724 amino acids,and the predicted molecular mass and isoelectric point were 78.9 kD and 4.66,respectively.The PmAmy contained a conservative A domain (Thrs4-Ser410) and a C domain (Glu420-Ala196) of alpha amylase family.Homology analysis revealed that the PmAmy shared 47％-99％ identity to other known amylase sequences,and the phylogenetic tree showed that the PmAmy was closely related to Litopenaeus vannamei.The expression levels of PmAmy in hepatopancreas were significantly higher than those of the other tissues (P＜0.05).The PmAmy expression was found in five ovarian development stages.The expression level in yolky stage (stageⅣ) was the highest among the five stages,and was the lowest in ovogonium stage (stage Ⅰ).The expression levels of PmAmy showed no significantly difference in ovary development stages.Expression of PmAmy was detected in all tested growth stages,and the expression level in mysis was significantly higher than that in nauplius,zoea and post larval (P＜0.05).These results suggested that PmAmy might be associated with larval development in P.monodon.关键词
斑节对虾/α-淀粉酶/基因克隆/基因表达/幼体发育Key words
Penaeus monodon/α-Amylase/Gene cloning/Gene expression/Larval development分类
生物科学引用本文复制引用
杨其彬,李运东,江世贵,黄建华,姜松,邱丽华,朱彩艳,周发林..斑节对虾α-淀粉酶基因的克隆及其表达分析[J].水生生物学报,2017,41(6):1186-1192,7.基金项目
现代农业产业技术体系建设专项(CARS-48) （CARS-48）
广东省省级科技计划项目(2014B020202003) （2014B020202003）
广东省海洋与渔业科技推广专项(A201501A06) （A201501A06）
深圳市生物产业发展专项资金现代农业生物产业推广扶持计划项目(NYSW201400331010053)资助[Supported by China Agriculture Research System (CARS-48) （NYSW201400331010053）
Guangdong Province Project of China (2014B020202003) （2014B020202003）
Guangdong Oceanic and Fisheries Project of China (A201501A06) （A201501A06）
Shenzhen Biological Industry Development Project of China (NYSW201400331010053)] （NYSW201400331010053）
