水生生物学报2017，Vol.41Issue(6)：1193-1199,7.DOI:10.7541/2017.148
三疣梭子蟹HMGBa基因克隆及其应答不同病原入侵的表达特征
CLONING OF HMGBA IN PORTUNUS TRITUBERCULATUS AND ITS EXPRESSION IN RESPONDING TO BACTERIAL AND VIRAL INFECTIONS
摘要
Abstract
Portunus trituberculatus is an economically important aquaculture species in China.As crustacean,it is lack of adaptive immunity and can induce effective immune responses to resist pathogen relying on innate immunity.Vibrio parahemolyticus and white spot syndrome virus (WSSV) have caused significant economic damage to P.trituberculatus aquaculture.High mobility group box (HMGB) proteins are known to be involved in diverse functions in mammalian cells.In crustacean,very limited studies on HMGB proteins have been documented.To investigate the role of high mobility group box (HMGB) proteins in innate immune system ofP.trituberculatus,the HMGB homologue cDNA from P.trituberculatus (named PtHMGBa) was first cloned by using the technology of rapid amplification of cDNA ends (RACE) in our study.The full-length cDNA of PtHMGBa was 1030 bp in length,which includes an open reading frame (ORF) of 681 bp,a 255 bp 3'untranslated region and a 94 bp 5'untranslated region.The ORF encoded a polypeptide of 227 amino acids with a predicted molecular weight of 25.82 kD.The polypeptide contained two HMG boxes domain and a C-terminal acidic tail composed of 24 rich Asp/Glu residues.The BLAST analysis showed that HMGB shared the highest homology with Litopenaeus vannamei HMCBa.The expression of PtHMGBa was mainly distributed in the haemocytes and hepatopancreas.A weak expression was detected in the eyestalk.Quantitative Real-time PCR analysis showed that PtHMGBa transcripts up-regulated significantly in haemocytes at 6h post V.Parahemolyticus inflection and up-regulated significantly in hepatopancreas at 48h post infection.Meanwhile,PtHMGBa transcripts significantly up-regulated in haemocytes at 12h post WSSV inflection,and the same result was observed in hepatopancreas.Our results suggested that PtHMGBa may play important roles in innate immunity of the crab,and would provide useful information for the research of immune regulation in P.trituberculatus and other crustaceans.关键词
三疣梭子蟹/高迁移率蛋白B/基因克隆/先天免疫/表达分析Key words
Portunus trituberculatus/HMGBa/Gene cloning/Innate immune/Expression analysis分类
农业科技引用本文复制引用
张杰,吕建建,刘萍,李健,王竹青,张小辉..三疣梭子蟹HMGBa基因克隆及其应答不同病原入侵的表达特征[J].水生生物学报,2017,41(6):1193-1199,7.基金项目
泰山领军人才工程高效生态农业创新类计划(LJNY2015002) （LJNY2015002）
国家自然科学基金面上项目(41506186) （41506186）
海洋渔业科学与食物产出过程功能实验室开放课题(2016LMFS-A12)资助[Supported by the Project of Taishan Scholars Leading Talent (LJNY2015002) （2016LMFS-A12）
the National Natural Science Foundation of China (41506186) （41506186）
Laboratory for Marine Fisheries Science and Food Production Processes,Qingdao National Laboratory for Marine Science and Technology,China (2016LMFS-A12)] （2016LMFS-A12）
