水生生物学报2017，Vol.41Issue(6)：1257-1264,8.DOI:10.7541/2017.156
基于线粒体CO Ⅰ基因序列的东南沿海可口革囊星虫遗传多样性分析
THE GENETIC DIVERSITY OF PHASCOLOSOMA ESCULENTA IN THE COASTAL ZONE OF SOUTH-EASTERN CHINA BASED ON SEQUENCE ANALYSIS OF MITOCHONDRIAL CO GENE
摘要
Abstract
To investigate the genetic diversity and structure ofPhascolosoma esculenta wild populations in the coastal zone of South-Eastern China,the mitochondrial cytochrome oxidase subunit Ⅰ (CO Ⅰ) genes as molecular markers were amplified,sequenced and analyzed from 80 organisms sampled in four areas [Xiangshan (XS),Wenling (WL),Ningde (ND) and Zhanjiang (ZJ)].The results showed that 815 base pair (bp) fragment was consisted of A,T,C and G base with 29.8％,31.0％,22.6％ and 16.6％,respectively,indicating a greater preference for A and T base because the A+T content (60.8％) was higher than that of G+C (39.2％).Besides,twenty-nine polymorphic sites and haplotypes were identified in all the sequences.Total haplotype diversity (Hd),nucleotide diversity (Pi) and the average number of nucleotide differences (K) were 0.932,0.0036 and 2.8902,respectively.A shallow topology of haplotype neighbor-joining (N J) tree with simple topological structure showed no phylogeographic structure among four populations.Genetic distance within and between P.esulenta populations were 0.0027-0.0040 and 0.0032-0.0040,respectively.Genetic fixation index (Fst) and analysis of molecular variance (AMOVA) indicated that the genetic variance mainly came from individuals within populations,and no genetic differentiation was among populations.Neutral test and mismatch-distribution analysis revealed that the populations ofP.esculenta had ever experienced population expansion that occurred in about 0.046 million years ago in the late pleistocene epoch.关键词
可口革囊星虫/CO Ⅰ基因/遗传多样性/遗传结构Key words
Phascolosoma esculenta/COgene/Genetic diversity/Genetic structure分类
生物科学引用本文复制引用
金丹璐,娄剑锋,高心明,侯聪聪,竺俊全,王建平..基于线粒体CO Ⅰ基因序列的东南沿海可口革囊星虫遗传多样性分析[J].水生生物学报,2017,41(6):1257-1264,8.基金项目
宁波市科技计划项目(2016C10004) （2016C10004）
浙江省重大科技专项重点农业项目(2011C12013) （2011C12013）
宁波大学海洋学院高级别科技成果奖培育计划资助[Supported by the Ningbo Science and Technology Plan Projects (2016C10004) （2016C10004）
the Major Science and Technology Projects in Zhejiang Province (2011C12013) （2011C12013）
the High-level Science and Technology Accomplishment Prize Cultivation Plan in School of Marine Sciences of Ningbo University] （）
