水生生物学报2017，Vol.41Issue(6)：1265-1272,8.DOI:10.7541/2017.157
基于核及叶绿体基因序列探讨中国绿水螅共生单细胞绿藻系统发生地位
THE SIGNIFICANCE OF PHYLOGENETIC OF UNICELLULAR GREEN ALGAE ENDOSYMBIOTIC IN HYDRA SINENSIS INFERRED FROM NUCLEAR AND CHLOROPLAST GENE SEQUENCES
摘要
Abstract
In the fields of evolutionary ecology,evolutionary biology and cell biology,symbiosis between Hydra sinensis and single-celled green algae is a special biological phenomenon with high scientific value.Although the biological significance of hydra-algea has been understood,its origin and evolutionary mechanism are still unclear by now.In the hydra-algae symbiosis status,free living algae goes into its host cell during evolution,so the study on the origin of symbiotic algae can help understand the mechanism of hydra-algae symbiosis.In this study,18S rRNA gene sequences in nucleolus and nine protein-coding genes (atpA,chlB,chlN,petA,psaB,psbA,psbC,psbD and rbcL) in chloroplast were cloned and sequenced from the symbiotic green algae in H.sinensis.With the application of Maximum Likelihood (ML) and Bayesian analysis methods,the significance of phylogenetic of the unicellular green algae endosymbiotic in H.sinensis was examined.The results show that the endosymbiotic green alga are belong to Chlorellales,Trebouxiophyceae,instead of Chlorellal;the green alga in paramecium,hydra,lichen and ginkgo are belong to Trebouxiophyceae,whereas others from frog and salamander are in Chlorophyceae.However,these two groups of endosymbiotic alga could live in the same host without forming a monophyletic group.In addition,endosymbiotic alga from different host cells have different origins.关键词
中国绿水螅/共生单细胞绿藻/系统发生/核18S rRNA基因/叶绿体基因Key words
Hydra sinensis/Symbiotic unicellular green algae/Phylogeny/Nuclear 18S rRNA gene/Chloroplast gene分类
生物科学引用本文复制引用
陈磊磊,刘俊红,李全发,董文芳,张行,潘红春..基于核及叶绿体基因序列探讨中国绿水螅共生单细胞绿藻系统发生地位[J].水生生物学报,2017,41(6):1265-1272,8.基金项目
国家自然科学基金(31370406和31671529)资助[Supported by the National Natural Science Foundation of China (31370406,31671529)] （31370406和31671529）
