水生生物学报2017，Vol.41Issue(6)：1311-1317,7.DOI:10.7541/2017.162
γ-氨基丁酸对鳜摄食和食欲的影响
EFFECTS OF γ-AMINOBUTYRIC ACID ON FOOD INTAKE AND APPETITE IN MANDARIN FISH SINIPERCA CHUATSI
摘要
Abstract
γ-Aminobutyric acid (GABA) has been established as an important regulator of food intake in mammals,but its role in fish remains scant.The aim of this study was to investigate the effects of GABA on food intake in mandarin fish (Siniperca chuatsi).Fish received intracerebroventricular (ICV) injection of saline and different doses of GABA (50,125,500 and 2000 μg).Food intake increased significantly in 125 μg GABA group within 2h.The RT-qPCR data demonstrated that up-regulation of NPY and AgRP,and downregulation of CART and POMC were consistent with higher food intake of mandarin fish.The mRNA expression of Leptin-R decreased significantly at all the four doses of GABA at 0.5h and 2h compared to the saline injected fish.These results indicated that GABA could affect the appetite by regulating leptin signaling pathway and thus resulting in alteration of food intake.Our findings could provide theory basis for GABA application in aquaculture feeds.关键词
γ-氨基丁酸/鳜/摄食/基因表达/leptin信号通路Key words
γ-Aminobutyric acid/Mandarin fish/Food intake/Gene expression/Leptin signaling pathway分类
农业科技引用本文复制引用
黄东,梁旭方,袁小琛,蔡文静,李艾璇,何珊,薛敏..γ-氨基丁酸对鳜摄食和食欲的影响[J].水生生物学报,2017,41(6):1311-1317,7.基金项目
Supported by the National Basic Research Program of China (2014CB138601) （2014CB138601）
the Fundamental Research Funds for the Central Universities (2015PY041) （2015PY041）
