中国生态农业学报2018，Vol.26Issue(3)：409-421,13.DOI:10.13930/j.cnki.cjea.170647
干旱条件下DCMU对高表达转C4-pepc水稻的花青素合成基因及其相关信号的影响
Effects of DCMU on anthocyanin synthesis genes and its related signals in C4-pepc gene overexpressed rice under drought conditions
摘要
Abstract
Anthocyanins are important antioxidant materials that protects plant from damage by reactive oxygen species (ROS). Especially under adverse conditions, the regulation of sucrose in plants depends on its ability to induce anthocyanin accumu-lation. To determine the intrinsic relationship between photosynthetic and anthocyanin regulated pathways for C4-phosphoenolpyruvate carboxylate (PEPC, EC 4.1.1.31) gene overexpressed rice (PC) in drought conditions, PC and un-transformed wild-type (WT) were treated with 50 μmol·L-1photosynthetic inhibitor DCMU for 1 h and the performance of the rice seedlings at 4-5 leaf stage observed under 12% PEG-6000 simulated drought. The results showed that DCMU pretreat-ment significantly reduced relative water contents of WT and PC under simulated 12% PEG-6000 drought condition, and rela-tive water content of PC was significantly higher than that of WT. The anthocyanin content was higher in PC than in WT under 12% PEG-6000 simulated drought or drought plus DCMU pretreatment. 12% PEG-6000 simulated drought decreased antho-cyanin contents of PC and WT, while DCMU pretreatment alleviated this effect. Compared with 12% PEG-6000, DCMU plus 12% PEG-6000 significantly inhibited net photosynthetic rate, stomatal conductance, intercellular CO2and carboxylation effi-ciency of the two rice lines, but these parameters of PC lines were significantly higher than those of WT lines. Then DCMU plus 12% PEG-6000 down-regulated endogenous sucrose content of the two materials, but sucrose content of PC lines was significantly higher than that of WT lines. Further studies showed that higher sucrose level in PC was associated with higher expression levels of transcriptional factors of bHLH (OsB1, OsB2), R2R3-MYB (OsC1), COP1 (constitutively photomorpho-genic 1),HY5(elongated hypocotyl 5),OsPAL,OsCHI,OsCHS,OsF3H,OsF3'H,OsDFR and OsANS,which resulted in syn-thesizing more anthocyanin to improve water retention capacity. In addition, PC rice sensed drought signals through NO and Ca2+, which participated in the regulation of transcription factors, regulation of anthocyanin synthesis gene, synthesis of more anthocyanin and thereby enhanced PC rice response to drought stress. This enhanced water retention capacity, stabilized pho-tosynthetic capacity and resisted drought. Therefore, it was beneficial in molecular breeding of "C4Rice" to study the sym-phony between high yield and plant resistance.关键词
转C4-pepc基因水稻/磷酸烯醇式丙酮酸羧化酶/蔗糖/花青素/DCMU/干旱Key words
C4-pepc gene overexpressed rice/Phosphoenolpyruvate carboxlase/Sucrose/Anthocyanin/DCMU/Drought分类
农业科技引用本文复制引用
何亚飞,许梦洁,李霞..干旱条件下DCMU对高表达转C4-pepc水稻的花青素合成基因及其相关信号的影响[J].中国生态农业学报,2018,26(3):409-421,13.基金项目
国家自然科学基金项目(31571585)、江苏省农业科学院基本科研业务专项(ZX[16]2002)和江苏省农业科学院粮食作物研究所基金(LZS17-9)资助This study was supported by the National Natural Science Foundation of China(31571585),the Special Project for Basic Research of Jiangsu Academy of Agricultural Sciences(ZX[16]2002)and the Grant from the Institute of Food Crops of Jiangsu Academy of Agriculture Sciences(LZS17-9). （31571585）
