生物骨科材料与临床研究2018，Vol.15Issue(1)：1-4,后插1,5.DOI:10.3969/j.issn.1672-5972.2018.01.001
过氧乙酸-乙醇联合辐照灭菌对脱钙骨基质成骨诱导活性的影响
Effects of peracetic acid-ethanol combined irradiation sterilization on the osteogenic inducing activity of demineralized bone matrix
摘要
Abstract
Objective To investigate the osteogenic activity ofdemineralized bone matrix (DBM) after Peracetic Acid -Ethanol (PES) combined irradiation sterilization.Methods The long bone of SD rats were divided into two groups to disinfection and inactivation:the experimental group which used PES solution add the cobalt-60γ radiation at 20kGy,and the control group just only used cobalt-60γ radiation at 20kGy.We made the DBM bone meal into capsules after disinfection,and implanted into SD rats,and the SD rats were sacrificed at 2,4,6,8 weeks,and remove the plant DBM bone from SD rat to detect the bone induction activity.Results From general observation,the DBM bone fragments from the experimental group were planted incomplete,scattered particles,on the contrary,experimental group disinfection planting had hard texture and complete shape.From new bone quantitative analysis,the results shows that the control group sterilized osteogenic activity is much better than the experimental group.From analysis of the sample of calcium,phosphorus,alkaline phosphatase enzyme content,the control group content is more than experimental group.Conclusion The osteogenic induction activity of DBM was reduced by using PES combined irradiation sterilization.关键词
骨植入材料/过氧乙酸-乙醇/脱钙骨基质/成骨诱导活性Key words
Bone Implant Materials/Peracetic Acid-Ethanol (PES)/Demineralized Bone Matrix (DBM)/Osteogenic Inducing Activity分类
医药卫生引用本文复制引用
李雄涛,余国荣,余黎,张旗,邓凯..过氧乙酸-乙醇联合辐照灭菌对脱钙骨基质成骨诱导活性的影响[J].生物骨科材料与临床研究,2018,15(1):1-4,后插1,5.基金项目
武汉市科技攻关项目(201161038344-05) （201161038344-05）
