生物骨科材料与临床研究2018，Vol.15Issue(1)：8-12,5.DOI:10.3969/j.issn.1672-5972.2018.01.003
压应力对人软骨终板细胞MMP-1、3、13和TIMP-1表达的影响研究
Effects of compressive stress on the expression of MMP-1, 3, 13 and TIMP-1 in human cartilage endplate cells
摘要
Abstract
Objective To investigate the effects of compressive stress on the secretion of matrix metalloproteinases-1,3,13 (MMP-1,MMP-3,MMP-13) and matrix metalloproteinase tissue inhibitor 1 (TIMP-1) in human cartilage endplate cells in vitro.Methods The cartilage endplate of 6 month aborted fetus was isolated and cultured.The second generation ofchondrocytes were cultured under the cyclic compressive stress (pressure size 0.8 MPa and frequency 0.1 Hz) with different duration (2 h,6 h and 12 h).Specimens were divided into group 2 h,group 6 h and group 12 h according to the duration of stress.The expression ofMMP-1,MMP-3,MMP-13 and TIMP-1 in the supematant of each group were detected.Results The endplate cells of the adherent wall appeared short fusiform,triangular and polygonal,typical of "cobblestone".With the increasing time of compressive stress,the spindle cell counts,the dedifferentiation phenomenon,the aging rate and the necrotic cell counts increased gradually.On the third day after stress,the expression of MMP-1,MMP-3 and MMP-13 were increased,while TIMP-1decreased in 6 h group and 12 h group.The longer the compression time,the more obvious the changes occurred in the 12 h group,these differences were statistically significant (P＜0.05) compared to other groups.Conclusion Long-term compressive stress increased the expression of MMP-1,MMP-3,MMP-13,decreased the expression of TIMP-1 and inhibited the proliferation of cells,and these changes were related to the duration of stress.关键词
压应力/软骨终板/基质金属蛋白酶/基质金属蛋白酶组织抑制因子Key words
Compressive stress/Cartilage endplate/MMP/TIMP分类
医药卫生引用本文复制引用
丁亮,唐果,薛锋,肖海军,何志敏,沈玉春..压应力对人软骨终板细胞MMP-1、3、13和TIMP-1表达的影响研究[J].生物骨科材料与临床研究,2018,15(1):8-12,5.基金项目
上海市卫生局青年基金课题(20134y138) （20134y138）
