辛伐他汀对非小细胞肺癌A549细胞增殖凋亡的影响及其机制OACSTPCD
Effect of simvastatin on proliferation and apoptosis of non-small cell lung cancer A549 cells and its mechanism
目的 观察辛伐他汀对非小细胞肺癌A549细胞生长状态的影响,探讨辛伐他汀作用下A549细胞发生凋亡的机制.方法 用0 μmol/L(对照组),10,20,30,40 μmol/L辛伐他汀分别对A549细胞进行处理,分别处理24,48,72 h,应用CCK-8法检测药物处理后A549细胞的增殖情况.辛伐他汀作用48 h,Hoechst33528荧光染色法、Annexin Ⅴ-FITC/PI双染法观察细胞形态学改变以及凋亡状况;蛋白印迹技术(West…查看全部>>
Objective To investigate the effect of simvastatin on the growth of non-small cell lung cancer A549 cells,and explore the mechanism of apoptosis induced by simvastatin in A549 cells. Methods A549 cells were treated with simvastatin at different concen-trations of 0 μmol/L(control group),10 μmol/L,20 μmol/L,30 μmol/L,40 μmol/L. After treated for 24 h,48 h and 72 h,the proliferation of A549 cells was detected by CCK-8 assay. After treatment for 48 h,Hoechst335…查看全部>>
方倩茹;梁冰;王效静;郦忆文
蚌埠医学院第一附属医院老年病科,蚌埠 233004蚌埠医学院第一附属医院老年病科,蚌埠 233004呼吸系病临床基础安徽省重点实验室蚌埠医学院第一附属医院老年病科,蚌埠 233004
医药卫生
辛伐他汀非小细胞肺癌A549细胞细胞增殖细胞凋亡
simvastatinnon-small cell lung cancerA549 cellscell proliferationapoptosis
《山西医科大学学报》 2018 (2)
118-122,5
安徽省中央引导地方科技发展专项项目(2016080503B035)
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