实用医学杂志2017，Vol.33Issue(22)：3706-3709,4.DOI:10.3969/j.issn.1006-5725.2017.22.007
茜草素介导BKCa通道beta亚基功能舒张肾叶间动脉降低SHR收缩压
Alizarin induced the function of beta subunits of BKCa channel relaxed interlobar renal artery and reduced SHR systolic pressure
摘要
Abstract
Objective To investigate the effect of alizarin on BKCa channel beta subunit and electrophysiological characteristics in interlobar renal artery of SHR.Methods Tail artery pressure measurement instrument,pressure myograph system,whole cell patch clamp technique and Western blot were used to measure the change of systolic pressure of SHR,renal interlobar arterial diameter,single vascular smooth muscle cell electrophysiological characteristics and expression of the BKCa channel beta subunit before and after alizarin intervention,respectively.Results (1) SHR systolic pressure was decreased after alizarin intervention (P ＜ 0.01).(2) ibTX inhibited alizarin-mediated renal interlobar arterial relaxation (P ＜ 0.01).(3) Alizarin enhanced BKCa channel-mediated outward currents (P ＜ 0.05).(4) Alizarin up-regulated BKCa channel beta subunits after alizarin intervention (P ＜ 0.01).Conclusion Alizarin reduced SHR systolic pressure and relaxed interlobar renal artery by enhancement BKCa currents via mediation of the function of beta subunits.关键词
茜草素/肾叶间动脉/BKCa通道/平滑肌细胞/全细胞膜片钳Key words
Alizarin/interlobar renal artery/BKCa channel/smooth muscle cell/whole cell patch clamp引用本文复制引用
章艳萍,王元媛,干艳捷,王家宁,张传林,李晓雯..茜草素介导BKCa通道beta亚基功能舒张肾叶间动脉降低SHR收缩压[J].实用医学杂志,2017,33(22):3706-3709,4.基金项目
国家自然科学基金资助项目(编号:81270221) （编号:81270221）
湖北省十堰市科技局资助项目(编号:15Y34,16K72) （编号:15Y34,16K72）
