天津中医药2018，Vol.35Issue(2)：143-146,4.DOI:10.11656/j.issn.1672-1519.2018.02.19
大黄素与丹参素合用对四氯化碳诱导大鼠肝纤维化的保护作用
Effects of combination of emodin and tanshinol in hepatic fibrosis rats induced by carbon tetrachloride
摘要
Abstract
[Objective]To study the effects of the combination of emodin and tanshinol in hepatic fibrosis rats with carbon tetrachloride (CCl4). [Methods] Fifty male SD rats were randomly divided into normal group, model group, emodin group, high dose emodin and tanshinol combination group, low dose emodin and tanshinol combination group. The hepatic fibrosis rat models in each group were induced by intraperitoneal injection of CCl4except normal group. Starting from the third week, in addition to injection molding, each experimental group was given corresponding drug gavage respectively, the normal group and model group were given intragastric administration of physiological saline for 6 weeks. After the last administration, blood and liver tissue were taken to observe the histopathological changes of the liver. The activities of serum alanin aspartate aminotransferase (AST), aminotransferase (ALT) and alkaline phosphatase (ALP)were measured.Levels of laminin (LN),hyaluronic acid (HA)and procollagenⅢ (PCⅢ)in serum were detected by enzyme linked immunosorbent assay (ELISA).The content of hydroxyproline (HYP)in liver homogenate was measured by acid hydrolysis method. [Results]Compared with the model group,the levels of ALT,AST,ALP,LN,HA,PCⅢand HYP liver tissue of each experimental group was significantly decreased (P<0.05,P<0.01),the liver pathological fibrosis was obviously relieve.Compared with emodin group, the levels of ALT, AST, ALP, LN, HA and HYP in high dose emodin and tanshinol combination group were significantly decreased (P<0.05 or P<0.01). [Conclusion]Emodin has antifibrotic effect;tanshinol can enhance the effect of emodin on antifibrotic effect.关键词
大黄素/丹参素/肝纤维化/肝功能/保护作用Key words
emodin/tanshinol/hepatic fibrosis/liver function/protective effect分类
医药卫生引用本文复制引用
吴晓东,张峰,梁瑞峰..大黄素与丹参素合用对四氯化碳诱导大鼠肝纤维化的保护作用[J].天津中医药,2018,35(2):143-146,4.基金项目
河南省科技攻关计划项目(162102310373) （162102310373）
河南省中医药科学研究专项课题(2015ZY2072). （2015ZY2072）
