天津中医药2018，Vol.35Issue(2)：147-150,4.DOI:10.11656/j.issn.1672-1519.2018.02.20
超高效液相色谱法同时测定金花葵花中5个黄酮类成分含量
Simultaneous determination of five flavonoids in the flowers of Aurea helianthus by ultra performance liquid chromatography method
摘要
Abstract
[Objective]To evaluate the quality of flowers of Aurea helianthus,ultra performance liquid chromatography (UPLC)method was established and validiated for simultaneous determining 5 flavonoids including rutin, hyperoside, isoquercitrin, myricetin and quercetin in Abelmoschusmanihot flowers.[Methods]The separation of 5 flavonoids was carried out on an ACQUITY UPLC BEH C18 (1.7 μm,2.1×100 mm)with gradient elutionusing acetonitrile and water containing 0.1% formic acid.The flow rate was 0.3 mL/min.The temperature was maintained at 35°C.The wavelength was recorded at 360 nm.[Results]All five flavonoids within their tested ranges showed good linear relationship with correlation coefficients which are greater than 0.999 0.The results showed good precisions,repeat abilities and stablities and the average recoveries were between 95% and 105%. The contents of five flavonoids existed striking differences among different harvest times and producing areas.[Conclusion]This fast,accurate and reliable method was could be used as tool for quantitative analysis of 5 flavonoids and evaluation of quality of flowers of Aurea helianthus.关键词
金花葵花/超高效液相色谱法/黄酮/含量测定Key words
flowers of Aurea helianthus/UPLC/flavonoids/quantification分类
医药卫生引用本文复制引用
白云,朱瑞超,李晋,马琳,何俊,常艳旭..超高效液相色谱法同时测定金花葵花中5个黄酮类成分含量[J].天津中医药,2018,35(2):147-150,4.基金项目
国家自然科学基金青年基金项目(81503213、81374050) （81503213、81374050）
大学生创新创业训练计划项目(201510063026). （201510063026）
