新疆农业科学2017，Vol.54Issue(10)：1775-1784,10.DOI:10.6048/j.issn.1001-4330.2017.10.002
利用mRNA差异显示技术筛选线果芥抗旱相关基因
Application of mRNA Differential Display Technique in Screening Drought Resistance Related Genes in C.planisiliqua
摘要
Abstract
[Objective] The ephemeral species Conringia planisiliqua belongs to Brassicaceae which grows in Xinjiang arid environment,which has strong drought resistance,but its drought resistance mechanism is not clear.In order to understand the mechanism of drought resistance and discover some excellent resistance genes from wild plant resources,it has laid an important foundation for further understanding the mechanism of drought resistance in conringia planisiliqua.[Method] Using the plant that has grown for three to four weeks treated by 20％ PEG6000,total RNA of the leaves at0 h,3 h,6 h,9 h,12 h,24 h and48 h was extracted.Then the differentially expressed genes were screened by mRNA differential display techniques.[Result] Eighty primer combinations were used to screen the differentially expressed cDNAs,and a total of 18 differentially expressed cDNAs were found.They were named of DF1-DF18,12 of the cDNAs were the up-regulated and 6 of them were down-regulated gene fragments.According to the gene function they were divided into 6 categories:basal metabolism,transcription factor,disease resistance protein,hypothetical protein,unknown protein and photoperiod protein.Most of them were the basal metabolism related genes.We analyzed the expression pattern of the DF-2,DF-6 and DF-14 under drought and salt stress,and the finding showed that the three genes were regulated by drought and salt.[Conclusion] Eighteen of the drought-resistant genes were screened from ephemeral plant Conringia planisiliqua.Three of the genes expression patterns were detected,they were induced by drought and salt stress.关键词
线果芥/抗旱/mRNA差异显示Key words
Conringia planisiliqua/drought stress/mRNA different display分类
农业科技引用本文复制引用
朱燕飞,陈全家,曲延英..利用mRNA差异显示技术筛选线果芥抗旱相关基因[J].新疆农业科学,2017,54(10):1775-1784,10.基金项目
国家转基因生物新品种重大专项“转基因耐旱、耐盐新品种培育”(2013ZX08005-004)National special program for new varieties of genetically modified organisms "Transgenic drought tolerance and salt tolerant new variety breeding" (2013ZX08005-004) （2013ZX08005-004）
