西南林业大学学报2018，Vol.38Issue(1)：28-33,6.DOI:10.11929/j.issn.2095-1914.2018.01.005
香榧种子抑制其发芽和生长的内源物筛选研究
Endogenous Screening of Torreya grandis Seeds Inhibitting Their Germination and Growth
摘要
Abstract
The GC-MS method was used to identify the species and relative content of endogenous in epi-sperm,endopleura,endosperm extracts of Torreya grandis seeds. At the same time,the bioassay was carried out u-sing the material of Brassica pekinensis as the acceptor,and the effect of endogenous substances from different parts of Torreya grandis seeds on the germination and growth inhibition of the Brassica pekinensis seed was analyzed. Re-sults showed that the organic compounds were mainly alkanes, phenols, alcohols, acids and so on. Episperm and endosperm ether phase contained 2,6-di-tert-butyl-p-cresol(relative content of 100%).The content of dimethylcy-clopentane in episperm and endosperm in petroleum ether phase were both higher than 13%,while the content en-dopleura was lower at 9.41%. The relative content of catechol was found to be 43.19% in the methanol phase ex-tract of endosperm. Seed germination was inhibited by extracts in the organic phase extracted from episperm,endo-pleura and episperm. The germiantion inhibitory effects conducted by episperm and endosperm were stronger than that by endopleura. Among them,the alkanes in the methanol phase extract of seed coat and catechol in the metha-nol phase extract of endosperm was highest inhibitory to the germination rate and seedling growth of Brassica pekin-ensis seedlings,and the inhibition rate was 100%. The petroleum ether phase of inside seed coat had no significant effect on the germination rate and growth of Brassica pekinensis seedlings.关键词
香榧/内源物/抑制/白菜种子/发芽Key words
Torreya grandis/endogenous material/inhibition/Brassica pekinensis seedlings/germination分类
农业科技引用本文复制引用
刘序,陈黎,高捍东..香榧种子抑制其发芽和生长的内源物筛选研究[J].西南林业大学学报,2018,38(1):28-33,6.基金项目
教育部博士点基金项目(20113204110010)资助. （20113204110010）
