西南林业大学学报2018，Vol.38Issue(1)：54-58,5.DOI:10.11929/j.issn.2095-1914.2018.01.009
钟花樱花粉离体萌发培养及低温保存技术研究
Pollen Germination in vitro Culture and Cryopreservation Technology of Cerasus campanulata
摘要
Abstract
The pollen of Cerasus campanulata were taken as material,aiming at studying the influence of pol-len germination on different concentrations of sucrose,boric acid,calcium chloride by in vitro method.And the pol-len preservation test was carried out under 3 kinds of low temperature conditions The result showed that the most suitable formula for pollen culture in vitro was 15%sucrose+50 mg/L boric acid+100 mg/L calcium chloride.Re-ducing the storage temperature can prolong the preservation of the pollen.Pollen almost dead after 30 days by stored in 4℃. The pollen germination rate was 28.30% after 150 days by stored in-20℃,and dead after 270 days. The pollen germination rate was 45.19% after 150 days by stored in -80℃,and dead after 270 days.关键词
钟花樱/花粉活力/培养液/离体培养/低温保存Key words
Cerasus campanulata/pollen viability/culture solution/in vitro culture/cryopreservation分类
农业科技引用本文复制引用
聂超仁,况红玲,于静亚,胡文洪..钟花樱花粉离体萌发培养及低温保存技术研究[J].西南林业大学学报,2018,38(1):54-58,5.基金项目
武汉市园林和林业局科研项目(武园林发[2017]49号)资助. （武园林发[2017]49号）
