西南林业大学学报2018，Vol.38Issue(1)：59-65,7.DOI:10.11929/j.issn.2095-1914.2018.01.010
杉木种鳞石蜡切片工艺优化研究
Process Optimization of Paraffin Section Method for Scale of Cunninghamia lanceolata
摘要
Abstract
In order to accurately observe the cell internal structure of Cunninghamia lanceolata scales, the processes,which was summarized by comparing the results of different treatments including 2 different fixing solu-tions,3 softening treatments,4 dehydrant and transparent treatments,time and temperature of waxing,embedding and dyeing,were optimized. Results showed that the material could be both fixed effectively by the FAA and FPA solutions;the best softening process was the hydrochloric acid solution for 12 h;the best dehydration time was 2 h per level,and the best transparent time was 1 h per level. Clear and complete cell structure map of the scales of C.lanceolata could be obtained by using this method.关键词
杉木/种鳞/石蜡切片/工艺优化Key words
Cunninghamia lanceolata/seed scale/paraffin section/process optimization分类
农业科技引用本文复制引用
左丹丹,熊升,李佳,饶丽莎,林思祖,陈宇..杉木种鳞石蜡切片工艺优化研究[J].西南林业大学学报,2018,38(1):59-65,7.基金项目
国家林业局杉木工程技术研究中心平台建设项目(ptjh130002)资助 （ptjh130002）
福建农林大学校重点项目建设专项(6112c0310)资助 （6112c0310）
林学学科建设项目(6112c0300)资助. （6112c0300）
