云南农业大学学报（自然科学）2017，Vol.32Issue(6)：1012-1021,10.DOI:10.16211/j.issn.1004-390X(n).2017.06.008
三七种子带菌检测及致病菌鉴定研究
Testing of Seed Borne Fungi of Panax notoginseng and Identification of Seed Pathogens
摘要
Abstract
[Purpose] the aim of this study is to determine the quantity and genus of fungi on the surface and inside of Panax notoginseng seeds,and identify the seed-associated pathogens.[Method] 15 seed samples were collected from Honghe and Wenshan City,and the fungi on the surface and inside of the seeds were isolated using PDA plate test and identified using morphology and molecular methods,meanwhile pathogenicity of isolates to the leaf,stem and root of P.notoginseng was evaluated.[Result] The results indicated that Sclerotinia sclerotiorum,Fusarium spp.,Botrytis spp.and Pestalotiopsis spp.were main fungi on the seed surface.Sclerotinia sclerotiorum,Fusarium spp.and Pestalotiopsis spp.were the main fungi inside seeds.Pathogenicity test showed that isolates of Fusarium spp.,Alternaria spp.and Bionectria spp.could infect leaf,stem and root of P.notoginseng,Gibberella intermedia,Fusarium tricinctum,F.moniliformis,and F.equiseti were the main seed-borne pathogens.[Conclusion] These results elucidated the main seed-borne pathogens of P.notoginseng are Fusarium spp.and Alternaria spp,this may provide theoretical basis for P.notoginseng seed treatment.关键词
三七/种传病原菌/镰刀菌/致病性测定Key words
Panax notoginseng/seed-borne pathogens/Fusarium/pathogenicity test分类
农业科技引用本文复制引用
赵芝,罗丽芬,郑建芬,何霞红,刘西莉,朱书生,杨敏..三七种子带菌检测及致病菌鉴定研究[J].云南农业大学学报（自然科学）,2017,32(6):1012-1021,10.基金项目
云南农业大学自然科学青年基金资助项目(2015ZR01) （2015ZR01）
云南省科技计划面上项目(2016FB148). （2016FB148）
