云南农业大学学报(自然科学)2017,Vol.32Issue(6):1022-1027,6.DOI:10.16211/j.issn.1004-390X(n).2017.06.009
烟草内源提取物抗TMV活性筛选
Extracts from Different Tobacco Varieties with Inhibitory Activity against Tobacco Mosaic Virus (TMV)
摘要
Abstract
[Purpose] The antiviral activities of tobacco extracts against tobacco mosaic virus (TMV) were studied to provide basis for exploitation of bio-pesticides from tobacco.[Method] The inhibitory activity against tobacco mosaic virus (TMV) was testing respectively with extracts from roots,stems,leaves and branches of Nicotiana glutinosa,Yunyan 203 and K326 distilled by the half-leaf method,and leaf disk method were used to confirm the validity of the results.[Result] The results showed that the extracts from roots and stems of this three tobacco varieties showed a good inhibitory activity against TMV.Especially,extracts from roots exhibited the best inhibitory activities and the ranges of inhibition rates were 65.00%-79.75%、64.08%-74.31% and 26.9%-42.40%;The inhibitory activities of the extracts from the stems were slightly weaker than the extracts from roots,but better than the extracts from leaves and branches,and the ranges of inhibition rates were 33.33%-44.25%,42.82%-66.06% and 10.96%-47.76%.The inhibition effect of the extracts from the leaves and branches of N.glutinosa,Yunyan 203 and K326 was not obvious.[Conclusion] The extracts from roots of this three tobacco varieties show a good inhibitory activity against TMV,which is valuable to study more.关键词
烟草/提取物/普通烟草花叶病(TMV)/抑制活性Key words
tobacco/extracts from tobacco/tobacco mosaic virus (TMV)/inhibitory activity分类
农业科技引用本文复制引用
周文兵,计思贵,李江舟,崔永和,张立猛..烟草内源提取物抗TMV活性筛选[J].云南农业大学学报(自然科学),2017,32(6):1022-1027,6.基金项目
中国烟草总公司云南省烟草公司科技计划项目(2015YN11). (2015YN11)